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1.1.1.42: isocitrate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about isocitrate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.42

Reaction

isocitrate
+
NADP+
=
2-oxoglutarate
 +
CO2
 +
NADPH
 +
H+

Synonyms

AbIDH1, AfIDH, CgIDH, cICDH, CtIDP1, CtIDP2, cytosolic isocitrate dehydrogenase 1, cytosolic NADP(+)-dependent isocitrate dehydrogenase, cytosolic NADP+-dependent isocitrate dehydrogenase, cytosolic NADP-dependent isocitrate dehydrogenase, cytosolic NADPH-dependent isocitrate dehydrogenase, DpIDH, EcIDH, HICDH, ICD, ICD1, ICD2, icdA, ICDH, ICDH-1, IDH, IDH-90, IDH-I, IDH-IIa, IDH-IIb, IDH1, IDH2, IDH3, IDHP, IDP, IDP1, IDP2, IDP3, IDPA, IDPc, IDPm, isocitrate dehydrogenase, isocitrate dehydrogenase (NADP), isocitrate dehydrogenase (NADP-dependent), isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate), isocitrate dehydrogenase 1, isocitrate dehydrogenase 2, isocitrate dehydrogenase-1, isocytrate deyhdrogenase, mICDH, mitochondrial NADP+-dependent isocitrate dehydrogenase, NAD+-dependent isocitrate dehydrogenase, NADP isocitric dehydrogenase, NADP+ dependent isocitrate dehydrogenase, NADP+-dependent Ds-threo-isocitrate dehydrogenase, NADP+-dependent Ds-threo-isocitrate:NADP+ oxidoreductase, NADP+-dependent ICDH, NADP+-dependent IDH, NADP+-dependent isocitrate dehydrogenase, NADP+-ICDH, NADP+-IDH, NADP+-isocitrate dehydrogenase, NADP+-linked isocitrate dehydrogenase, NADP+-specific ICDH, NADP+-specific IDH, NADP+-specific isocitrate dehydrogenase, NADP-dependent IDH, NADP-dependent isocitrate dehydrogenase, NADP-dependent isocitric dehydrogenase, NADP-ICDH, NADP-IDH, NADP-IDH Idp1p, NADP-isocitrate dehydrogenase, NADP-linked isocitrate dehydrogenase, NADP-specific isocitrate dehydrogenase, NADPH-dependent isocitrate dehydrogenase, NADPH-IDH, oxalosuccinate decarboxylase, oxalsuccinic decarboxylase, perICDH, PS-NADP-IDH, TaIDH, TM1148, YlIDP

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.42 isocitrate dehydrogenase (NADP+)

Purification

Purification on EC 1.1.1.42 - isocitrate dehydrogenase (NADP+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
41fold by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting
-
672fold to homogeneity
-
ammonium sulfate precipitation
by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting
-
Co2+ affinity resin column chromatography
cytosolic and mitochondrial isozymes
-
Flag-tag affinity column chromatography, and Superdex 200 gel filtration
glutathione Sepharose column chromatography
glutathione-Sepharose column chromatography and DEAE-Sepharose chromatography
His-tagged enzyme is purified by TALON resin column chromatography, ICDH fused maltose-binding protein is purified by amylose resin chromatography
His-Trap Ni-agarose column chromatography
isozymes ICDH1, 172fold, and ICDH2, 31fold, by ammonium sulfate fractionation, desalting, DEAE ion exchange and gel filtration
-
metal affinity resin column chromatography
-
NADP-isocitrate dehydrogenase from the normoxic and ischemic rat myocardium
native enzyme by PEG fractionation, affinity and hydrophobic interaction chromatography
-
native isozymes, partially
Ni-NTA affinity resin column chromatography
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and Sephacryl-S200 gel filtration
-
Ni-NTA resin column chromatography and Resource S column chromatography
Ni2+-Sepharose column chromatography
partially, isozyme ICD1
-
recombinant enzyme from Escherichia coli by ion exchange and gel filtration to homogeneity, 1.84fold
-
recombinant enzyme variants fused to the maltose binding fusion protein, from Escherichia coli, to homogeneity by amylose affinity, and ion exchange chromatography
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography
recombinant His-tagged IDPA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged isozymes IDP1 and IDP2 by nickel affinity chromatography
-
recombinant His-tagged mutant enzymes C269S and C379S from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant IDH1s from Escherichia coli by nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3)
-
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by Co2+ affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) b metal affinity chromatography
recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography and gel filtration
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant solubilized enzyme by ultrafiltration, and DEAE ion exchange chromatography, 6.4fold
Q8X277
recombinant soluble enzyme from Escherichia coli
-
recombinant wild-type and mutant enzymes from Escherichia coli, cleavage of the fusion proteins by thrombin, to homogeneity by amylose affinity chromatography
-
recombinant wild-type and mutant enzymes from Escherichia coli, to homogeneity
recombinant wild-type and mutant enzymes from Escherichia coli, to homogeneity by amylose affinity chromatography, thrombin digestion, and DEAE ion exchange chromatography
-
recombinant wild-type and mutant maltose binding fusion proteins from Escherichia coli by amylose affinity and size exclusion chromatography
-
strain ABE-1
-
TALON metal affinity resin column chromatography
wild-type and mutant enzymes