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0.013 - 40
2-oxoglutarate
0.00335 - 1930
DL-isocitrate
additional information
additional information
-
0.013
2-oxoglutarate
mutant enzyme R100Q, at pH 7.5 and 21°C
0.028
2-oxoglutarate
mutant enzyme R100Q, at pH 7.5 and 37°C
0.04
2-oxoglutarate
wild type enzyme, at pH 7.5 and 37°C
0.08
2-oxoglutarate
mutant enzyme R132N, at pH 7.5 and 37°C
0.24
2-oxoglutarate
mutant enzyme R132H, at pH 7.5 and 21°C
0.3
2-oxoglutarate
mutant enzyme R153H, at pH 7.5 and 25°C
0.45
2-oxoglutarate
mutant enzyme R153C, at pH 7.5 and 25°C
0.659
2-oxoglutarate
mutant enzyme R132W, at pH 7.5 and 37°C
0.9
2-oxoglutarate
mutant enzyme R132K, at pH 7.5 and 37°C
1
2-oxoglutarate
mutant enzyme R132H, in potassium phosphate buffer, at pH 7.5 and 37°C
1.5
2-oxoglutarate
mutant enzyme R132G, at pH 7.5 and 21°C
2.1
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C
2.3
2-oxoglutarate
mutant enzyme R132C, at pH 7.5 and 21°C
3.4
2-oxoglutarate
mutant enzyme R132A, at pH 7.5 and 37°C
3.8
2-oxoglutarate
mutant enzyme R132H, at pH 7.5 and 37°C
4.1
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C
4.4
2-oxoglutarate
mutant enzyme R132C, at pH 7.5 and 37°C
5
2-oxoglutarate
mutant enzyme R132G, at pH 7.5 and 37°C
5.8
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C
6
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C
7.5
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C
8.3
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C
13
2-oxoglutarate
wild type enzyme, in Tris buffer, at pH 7.2 and 37°C
14
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C
15
2-oxoglutarate
wild type enzyme, in Tris buffer, at pH 7.5 and 37°C
18
2-oxoglutarate
mutant enzyme R132Q, at pH 7.5 and 37°C
24
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 6.8 and 37°C
27
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C
27
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C
40
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C
0.00335
DL-isocitrate
with Mg2+, pH 7.5, 50°C
0.00348
DL-isocitrate
with Mn2+, pH 7.5, 50°C
335
DL-isocitrate
with Mg2+, pH 7.5, 37°C
348
DL-isocitrate
with Mn2+, pH 7.5, 37°C
1930
DL-isocitrate
-
at pH 7.4 and 37°C
0.02
isocitrate
mutant enzyme R132H, at pH 7.5 and 21°C
0.031
isocitrate
mutant enzyme R132N, at pH 7.5 and 37°C
0.074
isocitrate
mutant enzyme A134D, at pH 7.5 and 21°C
0.16
isocitrate
mutant enzyme R100Q, at pH 7.5 and 21°C
0.28
isocitrate
mutant enzyme R132G, at pH 7.5 and 21°C
0.29
isocitrate
mutant enzyme A134D, at pH 7.5 and 37°C
0.3
isocitrate
mutant enzyme R132C, at pH 7.5 and 21°C
0.34
isocitrate
mutant enzyme R132W, at pH 7.5 and 37°C
0.54
isocitrate
mutant enzyme R132C, at pH 7.5 and 37°C
0.57
isocitrate
mutant enzyme R132H, at pH 7.5 and 37°C
0.7
isocitrate
mutant enzyme R100Q, at pH 7.5 and 37°C
1.2
isocitrate
mutant enzyme D273N, in potassium phosphate buffer, at pH 7.5 and 37°C
1.3
isocitrate
mutant enzyme D273S, in potassium phosphate buffer, at pH 7.5 and 37°C
1.3
isocitrate
mutant enzyme R132G, at pH 7.5 and 37°C
1.8
isocitrate
mutant enzyme R132A, at pH 7.5 and 37°C
2
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G
3.7
isocitrate
mutant enzyme D273L, in potassium phosphate buffer, at pH 7.5 and 37°C
6
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A
7
isocitrate
mutant enzyme R132K, at pH 7.5 and 37°C
8
isocitrate
mutant enzyme R153H, at pH 7.5 and 25°C
9
isocitrate
mutant enzyme R153C, at pH 7.5 and 25°C
12
isocitrate
mutant enzyme R132Q, at pH 7.5 and 37°C
13
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T
35
isocitrate
mutant enzyme H133Q, at pH 7.5 and 21°C
45.4
isocitrate
mutant enzyme C3636S, at pH 7.5 and 25°C
49.7
isocitrate
wild type enzyme, at pH 7.5 and 25°C
83
isocitrate
mutant K413Q of isoform IDH2, at pH 7.4 and 25°C
88
isocitrate
mutant K256Q of isoform IDH2, at pH 7.4 and 25°C
110
isocitrate
mutant enzyme H133Q, at pH 7.5 and 37°C
111
isocitrate
wild type isoform IDH2, at pH 7.4 and 25°C
115
isocitrate
mutant enzyme K217Q, in potassium phosphate buffer, at pH 7.5 and 37°C
250
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E
280
isocitrate
mutant enzyme K217M, in potassium phosphate buffer, at pH 7.5 and 37°C
300
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A
320
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H
350
isocitrate
wild type enzyme, in Tris buffer, at pH 7.2 and 37°C
390
isocitrate
wild type enzyme, at pH 7.5 and 37°C
430
isocitrate
wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C
480
isocitrate
wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C
480
isocitrate
wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C
500
isocitrate
pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme
590
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C
590
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 8.0 and 37°C
610
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C
620
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C
630
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C
680
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C
690
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C
700
isocitrate
60°C, pH 8.0
730
isocitrate
wild type enzyme, at pH 7.5 and 21°C
750
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C
1000
isocitrate
pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme
1000
isocitrate
wild type enzyme, in Tris buffer, at pH 8.0 and 37°C
1010
isocitrate
wild type enzyme, in Tris buffer, at pH 7.8 and 37°C
1164
isocitrate
-
wild type enzyme, at pH 8.0 and 20°C
1227
isocitrate
-
mutant enzyme A741S, at pH 8.0 and 20°C
1400
isocitrate
wild type enzyme, in Tris buffer, at pH 7.5 and 37°C
1914
isocitrate
-
mutant enzyme E596L, at pH 8.0 and 20°C
2100
isocitrate
wild type enzyme, at pH 7.5 and 25°C
2600
isocitrate
40°C, pH 8.0
3086
isocitrate
-
at pH 8.0 and 20°C
6810
isocitrate
-
wild type enzyme, at pH 8.0 and 20°C
101970
isocitrate
-
mutant enzyme L594E, at pH 8.0 and 20°C
0.01996
NAD+
mutant enzyme H590L/R601D, in the presence of Mn2+, at pH 8.2 and 45°C
0.03278
NAD+
mutant enzyme H590L/R601D/R650S, in the presence of Mn2+, at pH 8.2 and 45°C
0.95
NAD+
pH 8.0, 25°C, recombinant mutant D253A/S257K/K260Q/R314D/H315I/T327A
3
NAD+
pH 8.0, 25°C, recombinant wild-type enzyme
4
NAD+
pH 8.0, 25°C, recombinant mutant D253A/S257K/K260Q/R314D/H315I/T327A
7
NAD+
-
wild type enzyme, at pH 8.0 and 25°C
9
NAD+
-
mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C
10
NAD+
-
mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C
20
NAD+
-
mutant enzyme H590L/R601L, at pH 8.0 and 25°C
23
NAD+
-
mutant enzyme H590L/R601L, at pH 8.0 and 25°C
1870
NAD+
pH 8.0, 25°C, recombinant wild-type enzyme
96500
NAD+
-
wild type enzyme, at pH 8.0 and 25°C
0.00553
NADP+
mutant enzyme H590L/R601D/R650S, in the presence of Mn2+, at pH 8.2 and 45°C
0.00613
NADP+
with Mn2+, pH 7.5, 50°C
0.00787
NADP+
with Mg2+, pH 7.5, 50°C
0.109
NADP+
mutant enzyme H590L/R601D, in the presence of Mn2+, at pH 8.2 and 45°C
1
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E
1.1
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A
1.3
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H
1.75
NADP+
pH 7.5, 25°C, recombinant mutant R322D, with Mg2+
3.303
NADP+
wild type enzyme, in the presence of Mn2+, at pH 8.2 and 45°C
4
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G
16
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A
33
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T
59
NADP+
mutant K413Q of isoform IDH2, at pH 7.4 and 25°C
186
NADP+
wild type isoform IDH2, at pH 7.4 and 25°C
205
NADP+
mutant K256Q of isoform IDH2, at pH 7.4 and 25°C
300
NADP+
at pH 8.5 and 42°C
613
NADP+
with Mn2+, pH 7.5, 37°C
787
NADP+
with Mg2+, pH 7.5, 37°C
1220
NADP+
pH 7.5, 25°C, recombinant wild-type enzyme, with Mg2+
1300
NADP+
pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme
1700
NADP+
pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme
5620
NADP+
-
at pH 7.4 and 37°C
additional information
additional information
turnover numbers of wild-type and chimeric enzymes
-
additional information
additional information
-
turnover numbers of wild-type and chimeric enzymes
-
additional information
additional information
turnover numbers of wild-type and chimeric enzymes
-
additional information
additional information
-
turnover numbers of wild-type and chimeric enzymes
-