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1.1.1.41: isocitrate dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about isocitrate dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.1.1.41

Reaction

isocitrate
+
NAD+
=
2-oxoglutarate
+
CO2
+
NADH
+
H+

Synonyms

At2g17130, At4g35260, At4g35650, At5g03290, AtIDH I, AtIDH II, AtIDH V, AtIDH VI, beta-decarboxylating dehydrogenase, beta-ketoglutaric-isocitric carboxylase, CaIDH, CcIDH, hNAD-IDHalpha, homodimeric NAD+-linked isocitrate dehydrogenase, Icd-2, ICDH, ICDH1, IDH, IDH I, IDH II, IDH III, IDH IV, IDH V, IDH-50, IDH-I, IDH-II, IDH-V, IDH1, IDH2, IDH3, IDH3A, IDH3alpha, IDH5, IDHa, isocitrate dehydrogenase, isocitrate dehydrogenase 2, isocitrate dehydrogenase 3alpha subunit, isocitrate-homoisocitrate dehydrogenase, isocitric acid dehydrogenase, isocitric dehydrogenase, McIDH, mIDH, MiIDH, mitochondrial NAD-dependent isocitrate dehydrogenase, monomeric NAD-IDH, NAD dependent isocitrate dehydrogenase, NAD isocitrate dehydrogenase, NAD isocitric dehydrogenase, NAD(P)+-dependent isocitrate dehydrogenase, NAD+-dependent ICDH, NAD+-dependent IDH, NAD+-dependent isocitrate dehydrogenase, NAD+-dependent isocitrate dehydrogenase 1, NAD+-dependent isocitrate dehydrogenase 3, NAD+-dependent type 1 IDH, NAD+-IDH, NAD+-specific ICDH, NAD+-specific IDH, NAD+-specific isocitrate dehydrogenase, NAD-Dependent IDH, NAD-dependent isocitrate dehydrogenase, NAD-ICDH, NAD-IDH, NAD-isocitrate dehydrogenase, NAD-linked isocitrate dehydrogenase, NAD-specific isocitrate dehydrogenase, NADH-IDH, OlIDH, OtIDH, PF0202, PH1722, Rv0066c, threo-DS-isocitrate:NAD+ oxidoreductase (decarboxylating), type 1 isocitrate dehydrogenase, type II homodimeric NAD-IDH, type II NAD+-specific isocitrate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.41 isocitrate dehydrogenase (NAD+)

Purification

Purification on EC 1.1.1.41 - isocitrate dehydrogenase (NAD+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
129fold to homogeneity by ammonium sulfate fractionation, hydrophobic chromatography on an octyl resin, and gel filtration
-
ammonium sulfate fractionation and gel filtration
-
Co2+ affinity column chromatography
-
gel filtration
-
Hi-Trap chelating column chromatography and Superdex 200 gel filtration
-
Hi-Trap chelating HP column chromatography
-
metal affinity resin column chromatography
-
mitochondrial preparation
-
multi-step purification of the native enzyme, 520fold to homogeneity, partial purification of the recombinant enzyme from Escherichia coli
Ni-NTA affinity resin column chromatography
-
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography and Sephacryl-S200 gel filtration
-
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni2+-affinity chromatography
Ni2+-nitrilotriacetate (Ni2+-NTA) column chromatography
Ni2+-nitrilotriacetic acid resin chromatography
-
Ni2+-nitrilotriacetic acid superflow resin chromatography
-
partially by mitochondrion isolation in a Percoll gradient, and ammonium sulfate precipitation in the 60-80% fraction, DEAE cellulose chromatography, and desalting
-
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold(DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from strain IDH12DELTAL by nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3)
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) by Co2+ affinity chromatography
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli
recombinant wild-type and mutant His6-tagged enzymes from Escherichia coli
recombinant wild-type and mutant NAD-IDHs from Escherichia coli by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration
-
TALON metal affinity resin column chromatography
-