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1.1.1.406: galactitol 2-dehydrogenase (L-tagatose-forming)

This is an abbreviated version!
For detailed information about galactitol 2-dehydrogenase (L-tagatose-forming), go to the full flat file.

Reaction

galactitol
+
NAD+
=
D-tagatose
+
NADH
+
H+

Synonyms

galactitol dehydrogenase, GatDH, More

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.406 galactitol 2-dehydrogenase (L-tagatose-forming)

Purification

Purification on EC 1.1.1.406 - galactitol 2-dehydrogenase (L-tagatose-forming)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His(6)-tagged GatDH from Escherichia coli strain BL21GOLD(DE3) by use of Co2+-IDA functionalized Fe2O3 nanoparticles, purification and immobilization method, overview
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His6-tagged GatDH from Escherichia coli strain BL21(DE3), by nickel affinity chromatography the tag is cleaved off, followed by dialysis and gel filtration
to homogeneity, chromatography steps
-