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1.1.1.37: malate dehydrogenase

This is an abbreviated version!
For detailed information about malate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.37

Reaction

(S)-malate
+
NAD+
=
oxaloacetate
 +
NADH
 +
H+

Synonyms

(R)-2-hydroxyacid dehydrogenase, (S)-malate dehydrogenase, A8B73_11505, CaMDH, cMDH, cMDH -S, cMDH-L, cyMDH, cyMDH1, cyMDH2, cyMDH3, cytMDH, cytMDH1, cytosolic malate dehydrogenase, cytosolic malate dehydrogenase 1, cytosolic MDH, cytosolic NAD-dependent malate dehydrogenase, flo16, floury endosperm16, halophilic malate dehydrogenase, HmMalDH, L-malate dehydrogenase, L-malate dehydrogenases, L-malate-NAD oxidoreductase, L-malate-NAD-oxidoreductase, L-malate: NAD oxidoreductase, L-malate: NAD+ oxidoreductase, L-malate:NAD oxidoreductase, L-malate:NAD-oxidoreductase, L-MDH, LDH-like malate dehydrogenase, ldh0076, LEUM_0076, m-MDH, malate (NAD) dehydrogenase, malate dehydrogenase, malate dehydrogenase (NAD), malate dehydrogenase 1, malate dehydrogenase 2, malate: NAD oxidoreductase, MalDH, malic acid dehydrogenase, malic dehydrogenase, mbNAD-MDH, MDH, MDH A, MDH B1, MDH B2, Mdh1, MDH2, Mdh2a, Mdh2b, Mdh3, mitochondrial malate dehydrogenase, mitochondrial MDH, mMDH, mNAD-MDH, More, NAD+ malate dehydrogenase, NAD+-dependent malate dehydrogenase, NAD+-dependent MDH, NAD+-MDH enzymes, NAD-dependent cytosolic malate dehydrogenase, NAD-dependent malate dehydrogenase, NAD-dependent MalDH, NAD-dependent malic dehydrogenase, NAD-dependent MDH, NAD-L-malate dehydrogenase, NAD-linked malate dehydrogenase, NAD-malate dehydrogenase, NAD-malic dehydrogenase, NAD-MDH, NAD-specific malate dehydrogenase, NADP+-dependent malate dehydrogenase, Pcal_1699, pdNAD-MDH, peroxisomal malate dehydrogenase, peroxisomal NAD+-malate dehydrogenase 1, peroxisomal NAD+-malate dehydrogenase 2, plastid-localized NAD-dependent MDH, plastidial NAD+-dependent malate dehydrogenase, plastidial NAD-dependent malate dehydrogenase, plastidial NAD-dependent malate dehydrogenase 1, plNAD-MDH, PMDH, PMDH1, PMDH2, regulatory subunit of nucleic acid-conducting channel, s-MDH, SrMalDH, TaMDH, VEG69, Vegetative protein 69, YlMdh2p, [LDH-like] MDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.37 malate dehydrogenase

Purification

Purification on EC 1.1.1.37 - malate dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes, cytoplasmic and mitochondrial
-
2 isoenzymes, S-MDH and M-MDH
-
ammonium sulfate fractionation and Cibacron Blue column chromatography
-
ammonium sulfate fractionation, CM-Sepharose column chromatography, and Blue Sepharose column chromatography
-
ammonium sulfate precipitation
ammonium sulfate precipitation, DEAE Toyopearl column chromatography, Sephadex G25 gel filtration, and Sephadex G200 gel filtration
-
both isoenzymes, sMDHase and mMDHase
-
DEAE cellulose column chromatography and Sephadex G-25 gel filtration
-
DEAE-Sephacelcolumn chromatography and Sephadex G-25 gel filtration
-
fusion protein of MDH with mitochondrial citrate synthase
-
glutathione-Sepharose column chromatography
-
HisTrap column chromatography and Superdex 200 gel filtration
native enzyme 103fold by two steps of anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration
-
native enzyme 103fold to homogeneity by two steps of anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration, recombinant His6-tagged enzyme by nickel affinity chromatography from Escherichia coli strain BL21(DE3)
-
native enzyme 478fold from seeds by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
-
native enzyme from leaves bound to plasma membranes by affinity chromatography
-
native enzyme from leaves, and two isozymes from roots bound to plasma membranes by affinity chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
native enzyme partially by subcellular fractionation
-
native tetrameric and dimeric MDH enzyme forms, 46 and 71fold, respectively, from mesophyll of maize leaves to homogeneity by ammonium sulfate fractionation, gel filtration, and anion excange chromatography
-
native two isozymes from inflorescences bound to plasma membranes by affinity chromatography
-
Ni-NTA agarose column chromatography and Sephacryl S-300 gel filtration
Ni-NTA column chromatography
-
Ni-Sepharose 6 column chromatography
-
Ni2+ Sepharose column chromatography, and Superdex 75 gel filtration
-
Ni2+-Sepharose column chromatography
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant enzyme
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography
recombinant His-Sumo-tagged enzyme from Escherichia coli stain BL21 (DE3) by nickel affinity chromatography, proteolytic cleavage of the His-Sumo tag, ultrafiltration, and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged MDH from Escherichia coli strain Rosetta(DE3) by cobalt affinity chromatography
recombinant N-terminal His-tagged cMDH from Escherichia coli strain BL21(DE3) pLysS inclusion bodies by affinity chromatography to over 95% purity
recombinant protein
-
recombinant soluble His6-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by gel filtration, hydrophobic interaction chromatography using 4 M KF and 1.3 M ammonium sulfate for elution, followed by dialysis
Source 30Q column chromatography and Superdex 75 gel filtration