1.1.1.359: aldose 1-dehydrogenase [NAD(P)+]
This is an abbreviated version!
For detailed information about aldose 1-dehydrogenase [NAD(P)+], go to the full flat file.
Reaction
Synonyms
gdh-1, glucose dehydrogenase, SsGDH, SSO3003
ECTree
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Substrates Products
Substrates Products on EC 1.1.1.359 - aldose 1-dehydrogenase [NAD(P)+]
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REACTION DIAGRAM
D-gluconate + NADH + 2 H+
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ir
beta-D-glucose + NAD+ + H2O
D-gluconate + NADH + 2 H+
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ir
beta-D-glucose + NAD+ + H2O
D-gluconate + NADH + 2 H+
kcat/Km for beta-D-glucose in the reaction with NAD+ is 1.4fold higher than the kcat/Km value for beta-D-glucose in the reaction with NADP+
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ir
beta-D-glucose + NAD+ + H2O
D-gluconate + NADH + 2 H+
when NAD+ serves as electron acceptor, D-glucose is oxidized at the highest rate among the substrates tested. No reverse reaction is evident, under various reaction conditions, when gluconic acid is used as substrate
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ir
beta-D-glucose + NAD+ + H2O
D-gluconate + NADH + 2 H+
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ir
D-gluconate + NADPH + 2 H+
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ir
beta-D-glucose + NADP+ + H2O
D-gluconate + NADPH + 2 H+
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ir
beta-D-glucose + NADP+ + H2O
D-gluconate + NADPH + 2 H+
it is likely that the first product of this reaction is delta-gluconolactone, which is readily converted into the acid. The lactone hydrolysis is facilitated by the high temperature and the slight alkaline pH value of the standard reaction conditions, thus explaining why no reverse reaction is observed under these conditions when delta-gluconolactone is used as substrate. A very slow NADH-dependent reduction of delta-gluconolactone by glucose dehydrogenase is observed at pH 7.0 and 37°C
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beta-D-glucose + NADP+ + H2O
D-gluconate + NADPH + 2 H+
kcat/Km for beta-D-glucose in the reaction with NADP+ is 1.4fold lower than the kcat/Km value for beta-D-glucose in the reaction with NAD+
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ir
beta-D-glucose + NADP+ + H2O
D-gluconate + NADPH + 2 H+
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?
beta-D-glucose + NADP+ + H2O
D-gluconate + NADPH + 2 H+
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ir
D-galactonate + NADH + 2 H+
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?
D-galactose + NAD+ + H2O
D-galactonate + NADH + 2 H+
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?
D-galactonate + NADPH + 2 H+
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D-galactose + NADP+ + H2O
D-galactonate + NADPH + 2 H+
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?
D-galactose + NADP+ + H2O
D-galactonate + NADPH + 2 H+
the activity is 70% of the activity with beta-D-glucose + NADP+
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the enzyme iss involved in aldose catabolism via the branched variant of the EntnerDoudoroff pathway
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additional information
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no activity with mannose or glucose 6-phosphate
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