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0.2 - 0.27
(S)-1,2,3,4-tetrahydronaphth-1-ol
0.035
3alpha-androstanediol
isoform AKR1C4, at pH 7.4 and 25°C
0.0038
4-androstenedione
pH 7.4, 37°C
0.0052 - 0.032
4-hydroxyandrostenedione
-
0.0078 - 0.025
4-hydroxytestosterone
-
0.004 - 0.367
5alpha-androstan-17-beta-ol-3-one
0.007
5alpha-Androstan-3alpha-ol-17-one
0.03
5alpha-androstane-3,17-dione
isoform AKR1C4, at pH 7.4 and 25°C
0.087 - 0.35
5alpha-androstane-3alpha,17beta-diol
0.003
5alpha-dihydroprogesterone
in 100 mM potassium phosphate, pH 7.0, at 25°C
0.033 - 0.1153
5alpha-dihydrotestosterone
0.0025 - 0.05
5alpha-pregnan-20alpha-ol-3-one
0.009 - 0.14
5beta-androstan-3,17-dione
0.004 - 0.18
5beta-Androstan-3alpha-ol-17-one
0.047 - 0.3
5beta-androstane-3alpha,17beta-diol
0.007 - 0.053
5beta-pregnane-3,20-dione
0.002 - 0.003
5beta-pregnane-3alpha,20alpha-diol
0.083 - 0.42
9alpha,11beta-prostaglandin F2
0.000017
allopregnanolone
in 100 mM potassium phosphate, pH 7.0, at 25°C
230
androstenol
reombinant His-tagged enzyme, pH 10.5, 25°C
0.032 - 0.13
chenodeoxycholic acid
0.89 - 48
Dehydrocholic acid
0.006 - 0.037
Dehydrolithocholic acid
0.012 - 0.085
deoxycholic acid
0.00075 - 0.026
dihydrotestosterone
0.032 - 0.043
lithocholic acid
0.0035 - 0.0392
pregn-4-en-3,20-dione
0.032
testosterone
pH 7.4, 37°C
additional information
additional information
Kcat-value for the 17beta-oxidation of testosterone is 0.000118 1/sec, and Kcat-value for the 17beta-reduction of androstenedione is 0.00112 1/sec
-
0.18
(R)-tetralol
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.38
(R)-tetralol
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.77
(R)-tetralol
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.2
(S)-1,2,3,4-tetrahydronaphth-1-ol
-
recombinant enzyme, at pH 7.4 and 25°C
0.27
(S)-1,2,3,4-tetrahydronaphth-1-ol
-
native enzyme, at pH 7.4 and 25°C
0.1
(S)-indan-1-ol
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.16
(S)-indan-1-ol
-
native enzyme, at pH 7.4 and 25°C
0.18
(S)-indan-1-ol
-
recombinant enzyme, at pH 7.4 and 25°C
0.27
(S)-indan-1-ol
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.4
(S)-indan-1-ol
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.2
(S)-tetralol
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.27
(S)-tetralol
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.43
(S)-tetralol
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.91
2-decalol
pH 10.5, 10°C
2.2
2-decalol
reombinant His-tagged enzyme, pH 10.5, 25°C
4.3
2-decalol
pH 10.5, 40°C
0.0052
4-hydroxyandrostenedione
pH 7.4, 37°C
-
0.017
4-hydroxyandrostenedione
pH 7.4, 37°C
-
0.0245
4-hydroxyandrostenedione
pH 7.4, 37°C
-
0.032
4-hydroxyandrostenedione
pH 7.4, 37°C
-
0.0078
4-hydroxytestosterone
pH 7.4, 37°C
-
0.01
4-hydroxytestosterone
pH 7.4, 37°C
-
0.025
4-hydroxytestosterone
pH 7.4, 37°C
-
0.004
5alpha-androstan-17-beta-ol-3-one
mutant F217S, pH 7.4, 25°C
0.367
5alpha-androstan-17-beta-ol-3-one
wild-type, pH 7.4, 25°C
0.007
5alpha-Androstan-3alpha-ol-17-one
-
native enzyme, at pH 7.4 and 25°C
0.007
5alpha-Androstan-3alpha-ol-17-one
-
recombinant enzyme, at pH 7.4 and 25°C
0.087
5alpha-androstane-3alpha,17beta-diol
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.135
5alpha-androstane-3alpha,17beta-diol
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.35
5alpha-androstane-3alpha,17beta-diol
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.033
5alpha-dihydrotestosterone
isoform AKR1C4, at pH 7.4 and 25°C
0.0612
5alpha-dihydrotestosterone
pH 7.4, 37°C, recombinant wild-type enzyme
0.1153
5alpha-dihydrotestosterone
pH 7.4, 37°C, recombinant mutant V54L
0.0025
5alpha-pregnan-20alpha-ol-3-one
mutant F217S, pH 7.4, 25°C
0.05
5alpha-pregnan-20alpha-ol-3-one
wild-type, pH 7.4, 25°C
0.009
5beta-androstan-3,17-dione
mutant F217S, pH 7.4, 25°C
0.14
5beta-androstan-3,17-dione
wild-type, pH 7.4, 25°C
0.004
5beta-Androstan-3alpha-ol-17-one
mutant F217S, pH 7.4, 25°C
0.048
5beta-Androstan-3alpha-ol-17-one
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.097
5beta-Androstan-3alpha-ol-17-one
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.113
5beta-Androstan-3alpha-ol-17-one
wild-type, pH 7.4, 25°C
0.18
5beta-Androstan-3alpha-ol-17-one
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.047
5beta-androstane-3alpha,17beta-diol
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.18
5beta-androstane-3alpha,17beta-diol
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.3
5beta-androstane-3alpha,17beta-diol
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.007
5beta-pregnane-3,20-dione
mutant F217S, pH 7.4, 25°C
0.053
5beta-pregnane-3,20-dione
wild-type, pH 7.4, 25°C
0.002
5beta-pregnane-3alpha,20alpha-diol
-
recombinant enzyme, at pH 7.4 and 25°C
0.003
5beta-pregnane-3alpha,20alpha-diol
-
native enzyme, at pH 7.4 and 25°C
0.083
9alpha,11beta-prostaglandin F2
-
recombinant enzyme, at pH 7.4 and 25°C
0.42
9alpha,11beta-prostaglandin F2
-
native enzyme, at pH 7.4 and 25°C
0.023
androsterone
isoform AKR1C4, at pH 7.4 and 25°C
0.043
androsterone
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.15
androsterone
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.28
androsterone
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
7.2
androsterone
pH 10.5, 40°C
500
androsterone
reombinant His-tagged enzyme, pH 10.5, 25°C
0.032
chenodeoxycholic acid
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.105
chenodeoxycholic acid
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.13
chenodeoxycholic acid
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.012
cholic acid
-
recombinant His-tagged mutant S114A/Y153F, pH 8.0, 37°C
0.03
cholic acid
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.045
cholic acid
-
recombinant His-tagged mutant K157A, pH 8.0, 37°C
0.05
cholic acid
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.2
cholic acid
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.62
cholic acid
-
recombinant His-tagged mutant Y153F, pH 8.0, 37°C
0.8
cholic acid
-
recombinant His-tagged mutant S114A, pH 8.0, 37°C
23
cholic acid
-
recombinant His-tagged wild-type enzyme, pH 8.0, 37°C
0.74
Cyclohexanol
pH 10.5, 40°C
2.5
Cyclohexanol
reombinant His-tagged enzyme, pH 10.5, 25°C
0.89
Dehydrocholic acid
-
recombinant His-tagged mutant S114A, pH 8.0, 37°C
1.1
Dehydrocholic acid
-
recombinant His-tagged mutant Y153F, pH 8.0, 37°C
48
Dehydrocholic acid
-
recombinant His-tagged wild-type enzyme, pH 8.0, 37°C
0.006
Dehydrolithocholic acid
mutant F217S, pH 7.4, 25°C
0.037
Dehydrolithocholic acid
wild-type, pH 7.4, 25°C
0.012
deoxycholic acid
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.018
deoxycholic acid
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.085
deoxycholic acid
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.00075
dihydrotestosterone
pH 7.4, 37°C
0.0175
dihydrotestosterone
pH 7.4, 37°C
0.023
dihydrotestosterone
pH 7.4, 37°C
0.025
dihydrotestosterone
pH 7.5, 37°C
0.026
dihydrotestosterone
pH 7.4, 37°C
0.032
lithocholic acid
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.04
lithocholic acid
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.043
lithocholic acid
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.2
NAD+
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.33
NAD+
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.58
NAD+
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.006
NADP+
mutant F217S, pH 7.4, 25°C
0.04
NADP+
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.16
NADP+
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.25
NADP+
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.72
NADP+
mutant N272T, pH 7.4, 25°C
0.85
NADP+
wild-type, pH 7.4, 25°C
0.01
NADPH
mutant F217S, pH 7.4, 25°C
0.077
NADPH
wild-type, pH 7.4, 25°C
0.1
NADPH
mutant N272T, pH 7.4, 25°C
0.0035
pregn-4-en-3,20-dione
pH 7.4, 37°C, recombinant wild-type enzyme
0.0392
pregn-4-en-3,20-dione
pH 7.4, 37°C, recombinant mutant V54L