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1.1.1.345: D-2-hydroxyacid dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about D-2-hydroxyacid dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.1.1.345

Reaction

an (R)-2-hydroxycarboxylate
+
NAD+
=
a 2-oxocarboxylate
+
NADH
+
H+

Synonyms

D-2-HADH, D-2-hydroxyacid dehydrogenase, D-2-hydroxyisocaproate dehydrogenase, D-HicDH, D-isomer specific 2-hydroxyacid dehydrogenase, D-isomer specific NAD+-dependent 2-hydroxyacid dehydrogenase, D-isomer-specific 2-hydroxyacid dehydrogenase, D-lactate dehydrogenase, D-lactate:NAD+ oxidoreductase, D-malate dehydrogenases, D-mandelate dehydrogenase, D-ManDH1, D-ManDH2, D-MDH, D2-HDH, HdhD, Ldb1010, ldh1837, LEUM_1837, ManDH2, NAD-dependent D-2-hydroxyacid dehydrogenase, PanE, R-HicDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.345 D-2-hydroxyacid dehydrogenase (NAD+)

Specific Activity

Specific Activity on EC 1.1.1.345 - D-2-hydroxyacid dehydrogenase (NAD+)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.427
-
with 2-oxoisocaproate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C
0.799
-
with 2-oxobutyrate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C
1.462
-
with pyruvate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C
170
-
after 77fold purification, in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C
2.2
-
crude extract, in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C
2.6
-
pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2
3.7
-
pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH1