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1.1.1.325: sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming)

This is an abbreviated version!
For detailed information about sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming), go to the full flat file.

Reaction

L-threo-7,8-dihydrobiopterin
+
NADP+
=
sepiapterin
+
NADPH
+
H+

Synonyms

MDSPR, sepiapterin reductase, SPR

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.325 sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming)

Purification

Purification on EC 1.1.1.325 - sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 533fold from cytosol by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration followed by affinity chromatography, another different step of anion exchange chromatography, and ultrafiltration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant His-tagged wild-type and selenomethionine-substituted enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, anion exchange chromatography, and gel filtration
-