1.1.1.318: eugenol synthase
This is an abbreviated version!
For detailed information about eugenol synthase, go to the full flat file.
Word Map on EC 1.1.1.318
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1.1.1.318
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phenylpropene
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phenylpropanoids
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ocimum
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basilicum
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scent
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emit
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petunia
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basil
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o-methyltransferase
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hybrida
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floral
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cinnamyl
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methyleugenol
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petals
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ripe
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pollinator
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benzenoid
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trichomes
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ammonia-lyase
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strawberry
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peltate
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estragole
- 1.1.1.318
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phenylpropene
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phenylpropanoids
- ocimum
- basilicum
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scent
-
emit
- petunia
- basil
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o-methyltransferase
- hybrida
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floral
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cinnamyl
- methyleugenol
- petals
-
ripe
-
pollinator
-
benzenoid
-
trichomes
-
ammonia-lyase
- strawberry
-
peltate
- estragole
Reaction
Synonyms
CbEGS1, CbEGS2, chavicol/eugenol synthase, DcE(I)GS1, DcEGS1, EGS, EGS1, EGS2, eugenol synthase 1, eugenol synthase 2, eugenol synthase 3, eugenol synthase 4, eugenol synthase 5, eugenol synthase 6, eugenol synthase 7, eugenol synthase 8, eugenol/isoeugenol synthase, IEGS1, IEGS2, LtCES1, More, ObEGS1, ObEGS2, ObEGS3, ObEGS4, ObEGS5, ObEGS6, ObEGS7, ObEGS8, PhEGS1, RcEGS1, RhEGS1
ECTree
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Substrates Products
Substrates Products on EC 1.1.1.318 - eugenol synthase
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REACTION DIAGRAM
chavicol + acetate + NADP+
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GC-MS analysis product analysis
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?
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
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r
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
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?
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
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?
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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CbEGS1 is specific for isoeugenol production
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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GC-MS analysis product analysis
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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GC-MS analysis product analysis
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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ObEGS1 uses a quinone methide intermediate-based mechanism to generate an intermediate to which the reductive transfer of the reducing hydride can then be easily accomplished. the enzyme acts on the substrate via a push-pull mechanism, removing the proton of the para hydroxyl group and promoting the cleavage of the acetyl group. In the resultant quinone-methide intermediate, the C7 atom serves as the acceptor of the hydride ion from NADPH
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coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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reaction via a quinone methide-like intermediate
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coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
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PhEGS1 converts coniferyl acetate to eugenol
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purified recombinant DcE(I)GS1 catalyzes the formation of eugenol (70% of total products, EC 1.1.1.318), as well as isoeugenol (30% of total products,, EC 1.1.1.319) from coniferyl acetate
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additional information
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Gymnadenia odoratissima proteins synthesize eugenol only
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additional information
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Gymnadenia odoratissima proteins synthesize eugenol only
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additional information
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Gymnadenia odoratissima proteins synthesize eugenol only
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additional information
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Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol
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additional information
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Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol
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additional information
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Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol
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additional information
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specificity of EGS for the production of allyl phenols in heterologically EGS expressing Fragaria x ananassa fruits
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additional information
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determinants of the regioselectivity of the EGS-catalyzed reduction reaction, overview
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additional information
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determinants of the regioselectivity of the EGS-catalyzed reduction reaction, overview
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additional information
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no substrate: 4-coumaryl acetate, cinnamyl acetate
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additional information
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GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
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GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
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GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
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GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
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GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
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GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
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GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
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GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
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no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
?
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no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
?
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no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
?
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no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
?
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no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
?
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no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
?
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no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
?
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no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
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additional information
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no substrate: cinnamyl acetate
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additional information
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no substrate: cinnamyl acetate
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additional information
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no substrate: cinnamyl acetate
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?