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1.1.1.318: eugenol synthase

This is an abbreviated version!
For detailed information about eugenol synthase, go to the full flat file.

Word Map on EC 1.1.1.318

Reaction

eugenol
+
a carboxylate
+
NADP+
=
a coniferyl ester
+
NADPH
+
H+

Synonyms

CbEGS1, CbEGS2, chavicol/eugenol synthase, DcE(I)GS1, DcEGS1, EGS, EGS1, EGS2, eugenol synthase 1, eugenol synthase 2, eugenol synthase 3, eugenol synthase 4, eugenol synthase 5, eugenol synthase 6, eugenol synthase 7, eugenol synthase 8, eugenol/isoeugenol synthase, IEGS1, IEGS2, LtCES1, More, ObEGS1, ObEGS2, ObEGS3, ObEGS4, ObEGS5, ObEGS6, ObEGS7, ObEGS8, PhEGS1, RcEGS1, RhEGS1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.318 eugenol synthase

Purification

Purification on EC 1.1.1.318 - eugenol synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native EGS 118fold from petals by alternating steps of anion exchange and hydrophobic interaction chromatography, co-purification with isoeugenol synthase, EC 1.1.1.319. Recombinant His-tagged CbEGS1 and CbEGS2 from Escherichia coli by nickel affinity chromatography
-
recombinant GST-taged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography and dialysis
-
recombinant GST-tagged enzyme EGS1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography