level of isoform Rdh11 is low and remarkably constant during development and oxidative stress. Rdh12 expression starts at postnatal day 7 and increases until postnatal day 30 to approximately sevenfold higher than Rdh11. Oxidative stress induced by exposure to constant bright light leads to a rapid and significant decrease of Rdh12 protein
RDH12 expression starts at postnatal day and increases until postnatal day 30. Oxidative stress induced by exposure to constant bright light leads to a rapid and significant decrease of RDH12 protein
Leydig cells, Sertoli cells, and germ cells: rdh11/12-like 1 and rdh11/12-like 3 transcripts are detected in all three fractions with no significant differences in expression level among the different fractions. In contrast, rdh11/12-like 2 mRNA is detected only in the germ cell fraction
quantitative RT-PCR expression analysis, expression pattern in different tissues. The pituitary and parts of brain and testis are the predominant sources of rdh11/12-like 1 transcript, while rdh11/12-like 2 mRNA is mostly detected in pituitary, parts of the brain, eye, and testis. Expression of rdh11/12-like 3, on the other hand, is ubiquitous across the tissues analyzed
RDH11 protein is most abundant in testis microsomes, with lower levels detectable in microsomes from liver, lung, and intestine. The rate of retinaldehyde reduction to retinol by the microsomes isolated from RDH11-null testis is 3fold lower compared with wild-type testis microsomes. Similarly to testis microsomes, liver microsomes lacking RDH11 show a lower rate (1.7fold) of retinaldehyde reduction. No differences are observed in the microsomal retinaldehyde reductase activities from livers of male versus female mice