1.1.1.292: 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)

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For detailed information about 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming), go to the full flat file.

Reaction

Synonyms

1,5-anhydro-D-fructose reductase, 1,5-anhydro-D-fructose reductase (ambiguous), AF reductase, AFR, AKR1E1, More, NADPH dependent 1,5-anhydro-D-fructose reductase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.292 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)

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Results	in table
1	Activating Compound
2454	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
6	Cofactor
2	Crystallization (Commentary)
2	Expression
2	General Information
30	KM Value [mM]
3	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
8	Organism
1	Pathway
1	PDB ID
3	pH Optimum
1	pH Range
1	pI Value
9	Protein Variants
2	Purification (Commentary)
1	Reaction
8	Reference
2	Specific Activity [micromol/min/mg]
1	Storage Stability
25	Substrates and Products (Substrate)
4	Subunits
17	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
17	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.292 - 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)

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native and recombinant enzyme

Ensifer adhaerens

Q2I8V6

667264

recombinant enzyme 17.6fold from Escherichia coli strain BL21 (DE3) by anion exchange and adsorption chromatography

Mus musculus

Q9DCT1

685692