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1.1.1.292: 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)

This is an abbreviated version!
For detailed information about 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming), go to the full flat file.

Reaction

1,5-anhydro-D-mannitol
+
NADP+
=
1,5-Anhydro-D-fructose
+
NADPH
+
H+

Synonyms

1,5-anhydro-D-fructose reductase, 1,5-anhydro-D-fructose reductase (ambiguous), AF reductase, AFR, AKR1E1, More, NADPH dependent 1,5-anhydro-D-fructose reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.292 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)

Cloned

Cloned on EC 1.1.1.292 - 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AKR1E1 gene, cloning from liver RNA, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21 (DE3)
expression in Escherichia coli
expresssion in Escherichia coli
gene afr, quantitative real-time RT-PCR enzyme expression analysis
overexpression in Escherichia coli