1.1.1.28: D-lactate dehydrogenase

This is an abbreviated version!
For detailed information about D-lactate dehydrogenase, go to the full flat file.

Reaction

Synonyms

D-(-)-lactate dehydrogenase, D-(-)-lactate dehydrogenase (NAD), D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein, D-lactate dehydrogenase, D-lactic acid dehydrogenase, D-lactic dehydrogenase, D-LDH, D-LDH-like enzyme, D-LDH0653, D-LDH1, D-LDH2, D-LDH3, D-LDH82319, D-nLDH, D-specific lactic dehydrogenase, dehydrogenase, D-lactate, DLD1, DLDH, DLDH744, ECBD_2243, ECLDH, FD35_GL001981, Fermentative lactate dehydrogenase, FN0511, FNLDH, lactic acid dehydrogenase, ldb0101, Ldb1010, LDH, LdhA, ldhd, LDHD1, LDHD2, LDHD3, LdhTi, Ljd-LDH, MGG_01202, NAD-dependent D-lactate dehydrogenase, PA0927, PALDH, Respiratory D-lactate dehydrogenase, SO_0968, tp0037, WP_013906894

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.28 D-lactate dehydrogenase

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Results	in table
10	Activating Compound
3400	AA Sequence
19	Application
1	CAS Registry Number
33	Cloned(Commentary)
168	Cofactor
15	Crystallization (Commentary)
6	Expression
29	General Information
5	General Stability
264	Inhibitors
115	kcat/KM [mM/s]
15	Ki Value [mM]
226	KM Value [mM]
9	Localization
21	Metals/Ions
33	Molecular Weight [Da]
112	Natural Substrates/ Products (Substrates)
17	Organic Solvent Stability
170	Organism
13	Pathway
43	PDB ID
52	pH Optimum
11	pH Range
9	pH Stability
2	pI Value
104	Protein Variants
32	Purification (Commentary)
11	Reaction
4	Reaction Type
156	Reference
31	Source Tissue
41	Specific Activity [micromol/min/mg]
8	Storage Stability
274	Substrates and Products (Substrate)
47	Subunits
129	Synonyms
1	Systematic Name
24	Temperature Optimum [°C]
8	Temperature Range [°C]
38	Temperature Stability [°C]
170	Turnover Number [1/s]

Application

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Application on EC 1.1.1.28 - D-lactate dehydrogenase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

biotechnology

Leuconostoc mesenteroides

P51011

construction of a metabolically engineered Saccharomyces cerevisiae that produces D-lactic acid efficiently. Two copies of the D-lactate dehydrogenase gene from Leuconostoc mesenteroides subsp. mesenteroides strain NBRC3426 are introduced into the genome. The D-lactate production reaches 61.5 g/l, the amount of glucose being transformed into D-lactic acid is 53.0% under non-neutralizing conditions. The D-lactic acid is of extremely high optical purity of 99.9% or higher

669543

diagnostics

Staphylococcus epidermidis

-

the enzyme is useful for selective determination of D,L-lactic acid in wines using peroxidase-based biosensors, method optimization, overview

688878

molecular biology

2 entries

synthesis

14 entries

additional information

Lactobacillus delbrueckii subsp. bulgaricus

-

protocol for undergraduate molecular biology and biochemistry laboratory courses spanning two semesters that is organized around the LdhA gene from the yogurt-fermenting bacterium Lactobacillus bulgaricus, using commercially available yogurt. Curriculum starts with cloning the LdhA gene into a prokaryotic expression vector, followed by mRNA isolation and characterization of recombinant gene expression levels using RT-PCR. The biochemistry module begins with overexpression of the cloned LdhA gene and guides students through the process of affinity purification, biochemical characterization of the purified LdhA protein, and analysis of enzyme kinetics using various substrates and an inhibitor, concluding with a guided inquiry investigation of structure-function relationships in the three-dimensional structure of LdhA using molecular visualization software

760538

molecular biology

Arabidopsis thaliana

-

D-lactate dehydrogenase is useful as a marker gene allowing positive selection of transgenic plants

722283

molecular biology

Arabidopsis thaliana Columbia-0

-

D-lactate dehydrogenase is useful as a marker gene allowing positive selection of transgenic plants

-

synthesis

Staphylococcus epidermidis

-

production of (R)-2-hydroxy-4-phenyl-butyric acid, which is a precursor for different ACE-inhibitors

659546

synthesis

Lactobacillus leichmannii

-

enzymatic synthesis of (R)-3,4-dihydrixyphenyllactic acid, a pharmacological compound that is used for the treatment of menstrual disorders, menostasis, menorrhalgia, insomnia, blood circulation diseases and Angina pectoris. Regeneration of NADH by formate dehydrogenase system. Use of genetic algorithm as a stochastic optimization method seems to be the best choice for the optimization

668233

synthesis

Thermodesulfatator indicus

F8A9V0

D-LDH is a candidate for thermophilic D-lactic acid production

740314

synthesis

Lactobacillus jensenii

-

the polymers of lactic acid are used as biodegradable bioplastics. Polylactic acid, biodegradable polyester polymer of lactic acid, is mainly produced through a bacterial fermentation process

739810

synthesis

Pediococcus pentosaceus

Q9AKS9

the recombinant enzyme from Pediococcus pentosaceus can be used for production of 3-phenyllactic acid (2-hydroxy-3-phenylpropanoic acid, PLA), an antimicrobial compound with broad spectrum activity against both bacteria and fungi, optimization by coexpression of Ogataea parapolymorpha formate dehydrogenase, EC 1.2.1.2, for NADH regeneration

740223

synthesis

Lactiplantibacillus plantarum

-

asymmetric synthesis of (R)-2-hydroxy-4-phenylbutyric acid using recombinant Pichia pastoris expressing the Tyr52Leu variant of D-lactate dehydrogenase from Lactobacillus plantarum. The recombinant yeast cells show catalytic activity at a high concentration of 2-oxo-4-phenylbutyric acid (380 mM, 76 g/l). Under optimized reaction conditions (pH 7.5, 37°C, and 2% glucose), a full conversion with over 95% reaction yield and about 100% product enantiomeric excess is achieved

760711

synthesis

Furfurilactobacillus rossiae

A0A0R1RJ30

recombinant Escherichia coli expressing D-lactate dehydrogenase, without coexpression of a cofactor regeneration system, can produce 20.5 g/l D-phenyllactic acid with enantiomeric excess above 99% from phenylpyruvic acid in a fed-batch biotransformation process, with a productivity of 49.2 g/l per day

760237

synthesis

Lactobacillus delbrueckii subsp. bulgaricus

P26297, Q1GAA2

recombinant Escherichia coli expressing Ldb0101 achieves a D-lactate concentration of 949.6 mg/l under aerobic and 1.94 g/l under anaerobic conditions, respectively

760242

synthesis

Lactobacillus delbrueckii subsp. bulgaricus

P26297, Q1GAA2

recombinant Escherichia coli expressing Ldb1010 achieves a D-lactate concentration of 850 mg/l

760242

synthesis

Furfurilactobacillus rossiae

A0A0R1RJ30

synthesis of D-phenyllactic acid by Escherichia coli expressing D-lactate dehdrogenase plus Exiguobacterium sibiricum glucose dehydrogenase. The total enzyme activity in the fermentation broth reaches 2359.0 U/l when induced by 10 g/l lactose at 28°C and 150 rpm for 14 h. Under the optimized biocatalysis conditions, 50 g/l sodium phenylpyruvate is completely converted to D-phenyllactic acid with a space-time yield and enantiomeric excess of 262.8 g/l day and over 99.5%, respectively

760239

synthesis

Lactobacillus delbrueckii subsp. bulgaricus DSM 20081

-

recombinant Escherichia coli expressing Ldb0101 achieves a D-lactate concentration of 949.6 mg/l under aerobic and 1.94 g/l under anaerobic conditions, respectively

-

synthesis

Lactobacillus delbrueckii subsp. bulgaricus DSM 20081

-

recombinant Escherichia coli expressing Ldb1010 achieves a D-lactate concentration of 850 mg/l

-

synthesis

Furfurilactobacillus rossiae DSM 15814

-

recombinant Escherichia coli expressing D-lactate dehydrogenase, without coexpression of a cofactor regeneration system, can produce 20.5 g/l D-phenyllactic acid with enantiomeric excess above 99% from phenylpyruvic acid in a fed-batch biotransformation process, with a productivity of 49.2 g/l per day

-

synthesis

Furfurilactobacillus rossiae DSM 15814

-

synthesis of D-phenyllactic acid by Escherichia coli expressing D-lactate dehdrogenase plus Exiguobacterium sibiricum glucose dehydrogenase. The total enzyme activity in the fermentation broth reaches 2359.0 U/l when induced by 10 g/l lactose at 28°C and 150 rpm for 14 h. Under the optimized biocatalysis conditions, 50 g/l sodium phenylpyruvate is completely converted to D-phenyllactic acid with a space-time yield and enantiomeric excess of 262.8 g/l day and over 99.5%, respectively

-