1.1.1.272: D-2-hydroxyacid dehydrogenase (NADP+)

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For detailed information about D-2-hydroxyacid dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

(R)-2-hydroxyacid dehydrogenase, (R)-sulfolactate dehydrogenase, (R)-sulfolactate:NAD(P)+ oxidoreductase, 2-D-hydroxyacid dehydrogenase, 2-HDH, 2HADH, D-2-hydroxyacid dehydrogenase, D-isomer specific 2-hydroxyacid dehydrogenase, D2-HDH, DDH, DDH_HALMT, HDH, L-2-hydroxyacid dehydrogenase, L-sulfolactate dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.272 D-2-hydroxyacid dehydrogenase (NADP+)

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Results	in table
507	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
8	Cofactor
3	Crystallization (Commentary)
18	General Information
13	KM Value [mM]
4	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
13	Organism
8	PDB ID
2	pH Optimum
3	Purification (Commentary)
3	Reaction
3	Reaction Type
12	Reference
1	Renatured (Commentary)
1	Specific Activity [micromol/min/mg]
54	Substrates and Products (Substrate)
4	Subunits
51	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Purification

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Purification on EC 1.1.1.272 - D-2-hydroxyacid dehydrogenase (NADP+)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

recombinant enzyme

Haloferax mediterranei

Q2VEQ7

710731

recombinant enzyme partially from Escherichia coli strain BL21(DE3) inclusion bodies by solubilization with 8 M urea in DTT-containing buffer and refolding by rapid 10fold dilution

Haloferax mediterranei

Q2VEQ7

672365

recombinant His6-tagged enzyme from Escherichia coli by nickel affinity and anion exchange chromatography, followed by gel filtration

Ketogulonicigenium vulgare

-

742168