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1.1.1.27: L-lactate dehydrogenase

This is an abbreviated version!
For detailed information about L-lactate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.27

Reaction

(S)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+

Synonyms

A4-LDH, AdhE, alpha-HBDH, alpha-hydroxybutyrate dehydrogenase, anaerobic lactate dehydrogenase, BbLDH, dehydrogenase, lactate, eLDHA, eLDHB, Epsilon crystallin, epsilon-crystallin, H4-L-lactate dehydrogenase, HBDH, heart LDH, Immunogenic protein p36, L(+)-nLDH, L-(+)-lactate dehydrogenase, L-lactate dehydrogenase, L-lactate dehydrogenase B, L-lactic acid dehydrogenase, L-lactic dehydrogenase, L-LDH, L-Ldh0845, L-nLDH, lactate dehydrogenase, lactate dehydrogenase 5, lactate dehydrogenase A, lactate dehydrogenase B, lactate dehydrogenase NAD-dependent, lactic acid dehydrogenase, lactic dehydrogenase, LctD, LDH, LDH-1, LDH-2, LDH-3, LDH-4, LDH-5, LDH-A, LDH-A4, LDH-m4, LDH1, LDH2, LdhA, LDHB, ldhL, LDHL1, LDHL2, LOX, LutACB, mL-LDH, mLDH, More, muscle LDH, NAD-dependent lactate dehydrogenase, NAD-lactate dehydrogenase, NGO0904, NGO0905, NGO0906, nitric oxideinducible l-lactate dehydrogenase, PfLDH, proteins, specific or class, anoxic stress response, p34, Sa-LDH-1, SMU.1115, SMU_1115, TeLdhL, Tsac_0416

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.27 L-lactate dehydrogenase

Purification

Purification on EC 1.1.1.27 - L-lactate dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
allozymes: LDH-Ba4, LDH-Ba/Bb and LDH-Bb4
-
by His bind purification
-
fructose 1,6-diphosphate-activated enzyme form
-
isoenzyme A4, B4 and X
-
isoenzyme I and II
-
isoenzyme LDH-5
Agama stellio stellio
-
isoenzyme LDH-A4 from muscle
-
isozymes LDHB and LDH 400fold and 30fold, from cell extracts of strains FI9078 and MG1363, respectively
native enzyme 34.3fold to about 98% purity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
native enzyme by ultracentrifugation, hydrophobic interaction chromatography, gel filtration, and ultrafiltration
native enzyme from malignant and healthy breast tissue 14.3fold by anion exchange and affinity chromatography
native enzyme to homogeneity from tail muscle of both aerobic control and anoxic crayfish 26.5fold and 69.4fold, respectively, by anion exchange chromatography and two steps of affinity chromatography
-
native enzyme, and recombinant His6-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3)
-
on cobalt affinity resin
optimization of a molecular analysis method based on the three-dimensional structure of protein and protein-ligand interactions using dye-ligand density for target protein separation, 3.7fold purification with expanded bed affinity adsorption, overview
-
partial
partially purified renal LDH
-
recombinant C-terminally His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(lambdaDE3) by centrfugation at 40000 g, nickel affinity chromatography, dialysis and ultrafiltration, followed by gel filtration, to over 95% purity
recombinant enzyme from Escherichia coli strain DH5alpha by heat treatment at 55°C for 90 s, hydrophobic interaction chromatography, hydroxyapatite chromatography, gel filtration, and ultrafiltration
recombinant enzyme from Escherichia coli strain DH5alpha by heat treatment at 70°C for 90 s, anion exchange chromatography, gel filtration, and ultrafiltration
recombinant enzyme from Escherichia coli strain Rosetta (DE3)pLysS by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography
-
recombinant His6-tagged enzyme from Escherichia coli BL21(DE3) by metal affinity chromatography dialysis, and ultrafiltration
recombinant His6-tagged LdhB from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography and cleavage of the tag
-
recombinant N-termially His6-tagged LDH-1 from Escherichia coli BL21 by nickel affinity chromatography and gel filtration to homogeneity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 by ion-exchange chromatography and affinity chromatography, respectively
-
to homogeneity yielding 18 mg of protein/litre culture
-
two major isoenzymes LDH1 and LDH2
Molinema dessetae
-
wild-type and enlarged loop mutants ELM1 and ELM2
-