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1.1.1.267: 1-deoxy-D-xylulose-5-phosphate reductoisomerase

This is an abbreviated version!
For detailed information about 1-deoxy-D-xylulose-5-phosphate reductoisomerase, go to the full flat file.

Word Map on EC 1.1.1.267

Reaction

2-C-methyl-D-erythritol 4-phosphate
+
NADP+
=
1-deoxy-D-xylulose 5-phosphate
+
NADPH
+
H+

Synonyms

1-deoxy-D-xylulose 5-phosphate isomeroreductase, 1-deoxy-D-xylulose 5-phosphate reductoisomerase, 1-deoxy-D-xylulose-5-phosphate, 1-deoxy-D-xylulose-5-phosphate reductoisomerase, 1-deoxy-D-xylulose-5-phosphate synthase, 1-deoxyxylulose 5-phosphate reductoisomerase, 1-deoxyxylulose-5-phosphate reductoisomerase, 2-C-methyl-D-erythritol 4-phosphate synthase, 2C-methyl-D-erythritol 4-phosphate synthase, 2C-methyl-D-erythritol-4-phosphate synthase, AaDXR, CaDXR, deoxyxylulose 5-phosphate reductoisomerase, deoxyxylulosephosphate reductoisomerase, DOXP reductoisomerase, DOXP-reductoisomerase, DXP reductoisomerase, DXP-reductoisomerase, DXR, DXR1, DzDXR, EcDXR, FtIspC, IspC, MEP synthase, meps, methylerythritol phosphate synthase, MtbIspC, MtDXR, Os01g01710, OsDXR, PdDXR, PfDXR, PtDXR, Rv2870c, SaDXR, VvDxr, YpIspC

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.267 1-deoxy-D-xylulose-5-phosphate reductoisomerase

Cloned

Cloned on EC 1.1.1.267 - 1-deoxy-D-xylulose-5-phosphate reductoisomerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic tree, functional complementation of Escherichia coli DXR-knockout mutants
DNA and amino acid sequence determination and analysis, quantitative expression analysis, expression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) periplasmic space
DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression pattern analysis, functional recombinant expression under the control of CaMV 35S promoter in Nicotiana tabacum cv. Zhongyan 90 via Agrobacterium-mediated transformation Overexpression of AvDXR in tobacco increases DXRactivity, photosynthetic pigment content and volatile isoprenoid components
dxr gene amplified and inserted into the pQE9 expression vector resulting in a protein with an amino-terminal His6-tag
-
expression in Escherichia coli
expression in Nicotiana tabacum
-
expression in tobacco as green fluorescent protein fusion protein
-
expression of an N-terminal His6-tagged enzyme variant comprising the first 389 of 413 residues
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli
-
gene DXR, DNA and amino acid sequence determination and analysis, cloning and promoter analysis of SaDXR, overview. Recombinant transient expression of full-length SaDXR fused to YFP in mesophyll protoplasts of Arabidopsis thaliana, recombinant overexpression in Arabidopsis thaliana cell lines using the Agrobacterium tumefaciens transfection method
MH018577
gene DXR, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant overexpression in Populus trichocarpa using the transfection method via Agrobacterium tumefaciens strain EHA105 transfecting young leaves and petioles of the Nanlin895 poplar, quantitative reverse transcription PCR enzyme expression analysis, the PtDXR gene is stably integrated into the genomes of the R1-1, R2-4, R3-1, R4-7, R5-3, R6-1, R7-3, and R8-2 lines and leads to expression of the DXR protein using the expression system of the poplar
gene dxr, DNA and amino acid sequence determination and analysis, sequence comparisons with other plant enzymes, quantitative real-time PCR enzyme expression analysis
gene DXR, phylogenetic analysis and tree, recombinant overexpression of DXR in Oryza sativa leaves and seeds, quantitative real-time PCR enzyme expression analysis
gene dxr, recombinant expression of tagged enzyme in Escherichia coli strain BL21 Codon Plus (DE3)-RIL
gene DXR, recombinant expression of YFP-tagged enzyme DXR in Catharanthus roseus cells
-
gene DXR, semi-quantitative RT-PCR enzyme expression analysis
-
gene DXR, sequence comparisons and phylogenetic analysis and tree, semi-quantitative RT-PCR and quantitative real-time PCR expression analysis, recombinant expression
gene dxr, sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ispC, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in the pseudomature form without plastid-targeting sequence in recombinant Escherichia coli
gene ispC, expression analysis, light-dependent expression pattern, overview
gene ispC, subcloning in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), Rv2870c complements an inactivated chromosomal copy of IspC in Salmonella enterica, and the complemented strain is sensitive to fosmidomycin
-
gene Rv2870c, expression of His-tagged enzyme in Escherichia coli strain BL21
-
gene Rv2870c, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
His6-tagged enzyme expression in Escherichia coli BL21-CodonPlus strain
-
into pMD-18T vector and transformed into Escherichia coli DH5alpha. Coding region of DXR cloned into vector pTrc, vectors pTrc-SmDXR and pAC-LYC cotransformed into the Escherichia coli DH5alpha
into the pQE31 expression vector resulting in an amino-terminal His6 fusion
-
into vector pMW118, expressed in Escherichia coli DYM1 transformant
into vector pQE30, ligation mixture electroporated into Escherichia coli XL1-Blue cells, giving the recombinant strains XL1-pQEispCMyco and M15-pQEispCMyco
-
overexpression and antisense downregulation in transformed peppermint plants
-
overexpression in Escherichia coli
overexpression in strain BL21 as His-tagged enzyme
-
overexpression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli
-
rapid amplification of cDNA ends
recombinant expression
-
recombinant expression of His-tagged enzyme
T87 cells expressing GFP-fused DXR. DXR transformed into Arabidopsis cells
-
transfection to Arabidopsis thaliana, expression in Escherichia coli mutants