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1.1.1.244: methanol dehydrogenase

This is an abbreviated version!
For detailed information about methanol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.244

Reaction

methanol
+
NAD+
=
formaldehyde
+
NADH
+
H+

Synonyms

activator-independent methanol dehydrogenase, ADH, BFZC1_05383, BsMdh, Bsph_4187, CNE_2c13570, dehydrogenase, methanol, group III NAD-dependent alcohol dehydrogenase, lxmdh, MDH, Mdh1, MDH2, Mdh3, MEDH, methanol dehydrogenase 2, More, NAD+ dependent methanol dehydrogenase, NAD-dependent MDH, NAD-dependent methanol dehydrogenase, XoxF

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.244 methanol dehydrogenase

Cloned

Cloned on EC 1.1.1.244 - methanol dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha
-
expressed in Escherichia coli, full activity only when grown in Mg2+ containing medium
-
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli. The expressed enzyme contains a 6-histidine tag at the N-terminus
-
gene BFZC1_05383, recombinant expression in Escherichia coli
-
gene Bsph_4187, recombinant expression in Escherichia coli
gene mdh, recombinant expression in Escherichia coli
gene mdh, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
I3DTM5, I3E2P9
gene mdh1, recombinant expression in Escherichia coli
gene mdh2 or CNE_2c13570, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain MG1655, recombinant expression of His-tagged enzyme Mdh2 in Escherichia coli strain XL-1
gene mdh2, recombinant expression in Escherichia coli
gene mdh2, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
I3DTM5, I3E2P9
gene mdh3, recombinant expression in Escherichia coli
methanol assimilation module is introduced into succinic acid producing Escherichia coli by employing the NAD-dependent methanol dehydrogenase from Bacillus methanolicus and ribulose monophosphate pathway from different donor organisms
recombinant enzyme expression in Escherichia coli
three isozymes, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain ER2566
-