1.1.1.227: (-)-borneol dehydrogenase
This is an abbreviated version!
For detailed information about (-)-borneol dehydrogenase, go to the full flat file.
Word Map on EC 1.1.1.227
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1.1.1.227
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monoterpene
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refolded
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bicyclic
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isozyme
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taiwan
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biotechnology
- 1.1.1.227
-
monoterpene
-
refolded
-
bicyclic
- isozyme
-
taiwan
- biotechnology
Reaction
Synonyms
BDH, BDH1, borneol dehydrogenase, EC 1.1.1.182, LiBDH, LiSDR-2, TCK1_3449, THL1_4180
ECTree
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Purification
Purification on EC 1.1.1.227 - (-)-borneol dehydrogenase
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native enzyme from strain TCU-HL1 by hydrophobic interaction and anion exchange chromatography, recombinant refolded enzyme from Escherichia coli
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partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns
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production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLysSby nickel affinity chromatography
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