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1.1.1.202: 1,3-propanediol dehydrogenase

This is an abbreviated version!
For detailed information about 1,3-propanediol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.202

Reaction

Propane-1,3-diol
+
NAD+
=
3-hydroxypropanal
+
NADH
+
H+

Synonyms

(NADH)-linked 1,3-PD oxidoreductase, 1,3-PD dehydrogenase, 1,3-PD oxidoreductase, 1,3-PD-DH, 1,3-PD:NAD+ oxidoreductase, 1,3-PDDH, 1,3-Pdiol dehydrogenase, 1,3-propanediol dehydrogenase, 1,3-propanediol oxidoreductase, 1,3-propanediol-oxidoreductase, 1,3-propanediol-oxydoreductase, 1,3-propanediol:NAD oxidoreductase, 3-hydroxypropionaldehyde reductase, ADH3, dehydrogenase, 1,3-propanediol, DhaT, lr_0030, lr_1734, NADH-dependent 1,3-PD dehydrogenase, NADH-dependent 1,3-propanediol oxidoreductase, NADH-linked 1,3-propanediol oxidoreductase, PDOR, YqhD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.202 1,3-propanediol dehydrogenase

Purification

Purification on EC 1.1.1.202 - 1,3-propanediol dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by centrifugation, nickel affinity column and gel filtration
Ni-NTA column chromatography
-
recombinant His-tagged enzyme 7.1-7.3fold from Escherichia coli strain JM109 by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant protein
-
recombinant soluble enzyme from Escherichia coli strain BL21(DE3)
-
recombinant tagged selenomethionine-labeled enzyme from Escherichia coli by nickel affinity, ion exchange, and gel filtration chromatography and ultrafiltration
soluble recombinant His-tagged enzyme 6.6-6.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity