1.1.1.201: 7beta-hydroxysteroid dehydrogenase (NADP+)

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For detailed information about 7beta-hydroxysteroid dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.201

1.1.1.201

synthesis

ursodeoxycholic

taurinates

dehydrogenases

nadph-dependent

aerofaciens

bile

Reaction

Synonyms

7beta-HSD, 7beta-HSDH, 7beta-hydroxysteroid dehydrogenase, More, NADP-dependent 7beta-hydroxysteroid dehydrogenase, RUMGNA_02585

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.201 7beta-hydroxysteroid dehydrogenase (NADP+)

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Results	in table
15	AA Sequence
5	Application
1	CAS Registry Number
6	Cloned(Commentary)
22	Cofactor
2	Crystallization (Commentary)
4	General Information
2	General Stability
2	Inhibitors
12	kcat/KM [mM/s]
39	KM Value [mM]
5	Localization
7	Molecular Weight [Da]
50	Natural Substrates/ Products (Substrates)
28	Organism
3	Pathway
2	PDB ID
12	pH Optimum
1	pH Range
19	Protein Variants
7	Purification (Commentary)
2	Reaction
3	Reaction Type
26	Reference
12	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
88	Substrates and Products (Substrate)
9	Subunits
32	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
7	Temperature Stability [°C]
17	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.201 - 7beta-hydroxysteroid dehydrogenase (NADP+)

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GST-Sefinose affinity column chromatography

[Ruminococcus] torques

760712

HisTrap column chromatography, Mono Q column chromatography, Mono S column chromatography and Superdex 75 gel filtration

Collinsella aerofaciens

760289

native enzyme from liver microsomes 300fold by ion exchange, hydroxylapatite, 2',5'-ADP affinity

Oryctolagus cuniculus

656659

recombinant His6-tagged enzyme from Escherichia coli strain JM109 by nickel affinity chromatography, dialysis, and ultrafiltration

[Ruminococcus] gnavus

A7B4V1

740835

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration

Collinsella aerofaciens

A4ECA9

741386

to homogeneity, chromatography techniques

2 entries