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1.1.1.188: prostaglandin-F synthase

This is an abbreviated version!
For detailed information about prostaglandin-F synthase, go to the full flat file.

Word Map on EC 1.1.1.188

Reaction

(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate
+
NADP+
=
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
 +
NADPH
 +
H+

Synonyms

17beta-hydroxysteroid dehydrogenase type 5, 17betaHSD5, 20alpha-HSD/PGFS, 20alpha-hydroxysteroid dehydrogenase/prostaglandin F-synthase, 9,11-endoperoxidase reductase, AKR1B1, AKR1B3, AKR1B7, AKR1C3, Akr1cl, aldo-keto reductase, aldo-keto reductase 1C3, aldo-ketoreductase 1B1, ALDR1, ALR2, DD1, dihydrodiol dehydrogenase 1, LBRM2904_31.2170, LemaA.00019.a.B1, m-PGFS, More, NADPH-dependent prostaglandin D2 11-keto reductase, P100/11E, PG endoperoxide reductase, PGD 11-ketoreductase, PGD2 11-ketoreductase, PGF, PGF synthase, PGF synthase I, PGF synthase II, PGF2alpha synthase, PGF2alpha-synthase, PGF2alpha-synthetic activity, PGF2S, PGFS, PGFS I, PGFS-I, PGFS-II, PGFSI, PGFSII, PGH 9-,11-endoperoxide reductase, PGH2 9,11-endoperoxidase, PGH2 9,11-endoperoxide reductase, PGH2 9-,11-endoperoxide reductase, prostaglandin 11-keto reductase, prostaglandin 11-ketoreductase, prostaglandin D2 11-ketoreductase, prostaglandin D2-ketoreductase, prostaglandin F synthase, prostaglandin F synthase I, prostaglandin F synthase II, prostaglandin F synthetase, prostaglandin F2alpha synthase, prostaglandin F2alpha-synthase, prostaglandin-D2 11-reductase, prostaglandin-F2alpha synthase, prostamide/PGF synthase, prostamide/prostaglandin F synthase, reductase, 15-hydroxy-11-oxoprostaglandin, synthetase, prostaglandin F2alpha, Tc00.1047053508461.80, Tc00.1047053511287.49, TCDM_00059, TrcrA.00019.a.B1, type 5 17beta-hydroxysteroid dehydrogenase/prostaglandin F synthase, vas deferens protein

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.188 prostaglandin-F synthase

Cloned

Cloned on EC 1.1.1.188 - prostaglandin-F synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence analysis, expression analysis in endometrium of cyclic and pregnant animals
expession in Escherichia coli MC1061 or HB101
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli HB101 cells
expression Escherichia coli HB101
expression in COS-7 cells
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli HB101
expression in Escherichia coli strain BL21(DE3) as GST-fusion protein
-
expression in Escherichia coli strain HB101
expression of myc-tagged GFP-PGFS in non-human primate eyes via FIV vector leading to a significant reduction of intraocular pressure, IOP, the GFP-tag can still be detected after 48-52 weeks, the decrease in IOP lasted for 5 months, method evaluation
-
gene AKR1B1, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression in immortalized human endometrial stromal cells, HIESC-2 cells, expression analysis in clones 16-1, 16-2, and 16-4. Clones 16-1 and 16-2 present with a complete absence of AKR1B1 protein, while clone 16-4 is able to express the AKR1B1 protein. Following treatment of cells with interleukin-1beta, both wild-type and clone 16-4 cells exhibit a similar response, but clone 16-2 does not respond with increased production of eitherPGF2alpha or PGE2. At the protein level, IL-1beta induces an increase in mPGES-1 and COX-2 protein expression but in clone 16-2 the relative increase of Cox-2 protein is weaker
gene AKR1B1, real-time PCR analysis enzyme expression
-
gene AKR1B1, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
gene AKR1C3, expression analysis
gene AKR1C3, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
gene P100/11E, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene PGFS, DNA and amino acid sequence determination and analysis
gene PGFS, quantitative expression analysis, recombinant expression of His-tagged canine-specific PGFS (AKR1C3) in Escherichia coli
gene Tc00.1047053511287.49, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
into vector pET-28a and expressed in Escherichia coli BL21 (DE3)
liver-type isozyme, full length cDNA from cDNA library, DNA and amino acid sequence determination and analysis, transient expression of wild-type and mutant enzymes in COS-7 cells
-
overexpression of AKR1C3 using a pLNCX retroviral vector in MCF-7 cell line
recombinant enzyme is expressed in Escherichia coli
-
SCC cell line is infected with either AKR1C3 overexpressing retroviral construct (SCC-AKR1C3), quantitative AKR1C3 expression analysis in wild-type and mtransfected cells
wild-type and mutants, plasmid pCold II-PM/PGFS expressed in Escherichia coli BL21 cells
-