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0.009
-
substrate 4-tert-butylacetophenone, mutant Q245L
0.013
-
substrate 4-bromoacetophenone, wild-type
0.014
-
substrate 4-fluoroacetophenone, wild-type
0.015 - 1.14
substrate specificity, overview
0.016
-
substrate 4-methoxyacetophenone, mutant Q245H
0.028
-
substrate acetophenone, wild-type
0.03
with cinnamaldehyde and NADH as substrates, at pH 7.0 and 30°C
0.032
-
substrate 4-tert-butylacetophenone, mutant Q245H
0.036
-
substrate 4-fluoroacetophenone, mutant Q245L
0.039
-
substrate acetophenone, mutant Q245P
0.045
-
substrate 4-methylacetophenone, mutant Q245P
0.047
-
substrate 4-bromoacetophenone, mutant Q245L
0.062
-
substrate 4-methoxyacetophenone, mutant Q245P
0.067
-
substrate 4-chloroacetophenone, mutant Q245L
0.072
-
substrate 4-fluoroacetophenone, mutant Q245H
0.073
-
substrate 1-phenylethanonee, pH 6.5, temperature not specified in the publication
0.074
-
substrate 1-(4-chlorophenyl)ethanone, pH 6.5, temperature not specified in the publication
0.084
-
substrate 4-tert-butylacetophenone, mutant Q245P
0.085
-
substrate acetophenone, mutant Q245H
0.086
-
substrate acetophenone, mutant Q245L
0.1
-
recombinant enzyme, substrate phenyl 3-oxobutyrate
0.12
-
recombinant enzyme, substrate trifluoromethyl 3-oxobutyrate
0.18
-
recombinant enzyme, substrate tert-butyl 3-oxobutyrate
0.2
-
recombinant enzyme, substrate ethyl 3-oxobutyrate
0.203
-
substrate 4-bromoacetophenone, mutant Q245H
0.238
-
substrate 4-chloroacetophenone, mutant Q245H
0.309
-
substrate 4-chloroacetophenone, mutant Q245P
0.403
-
substrate 4-bromoacetophenone, mutant Q245P
0.45
with 4-hydroxybenzaldehyde and NADPH as substrates, at pH 7.0 and 30°C
0.47
with 2-chlorobenzaldehyde and NADPH as substrates, at pH 7.0 and 30°C
0.48
-
crude extract, at pH 7.0 and 25°C
0.5
-
purified recombinant enzyme
0.522
-
substrate ethyl 3-oxopentanoate, pH 6.5, temperature not specified in the publication
0.6
substrate benzaldehyde, pH 6.0, 25°C
0.62
with 2-oxo-2-phenylacetic acid and NADPH as substrates, at pH 7.0 and 30°C
0.64
with ethyl-2-oxo-2-phenylacetate and NADH as substrates, at pH 7.0 and 30°C
0.65
substrate furfural, pH 6.0, 25°C
0.8
-
recombinant enzyme, substrate isopropyl 3-oxobutyrate
0.85
with ethyl-2-oxo-2-phenylacetate and NADPH as substrates, at pH 7.0 and 30°C
0.96
substrate methyl glyoxal, pH 6.0, 25°C
1.1
substrate p-anisaldehyde, pH 6.0, 25°C
1.4
-
recombinant enzyme, substrate chloromethyl 3-oxobutyrate
1.5
substrate hexanal, pH 6.0, 25°C
1.63
with 2-oxo-2-phenylacetaldehyde and NADPH as substrates, at pH 7.0 and 30°C
10.9
-
substrate 4-bromobenzaldehyde, pH 6.5, temperature not specified in the publication
125.3
Cylindrocarpon sclerotigenum
-
purified enzyme
13.35
with 4-nitrobenzaldehyde as substrate, at pH 7.5 and 30°C
1502
-
substrate ethyl 4-chloro-3-oxobutanoate, pH 6.5, temperature not specified in the publication
1533
substrate 2,3-butanedione, pH 7.4, 37°C
161.6
substrate daunorubicin, pH 7.4, 37°C
17
substrate 2,3-pentanedione, pH 7.0, 25°C
18.63
with 4-cyanobenzaldehyde as substrate, at pH 7.5 and 30°C
19.1
substrate phenylglyoxal, pH 7.0, 25°C
19.29
with pentanal as substrate, at pH 7.5 and 30°C
19.69
with 4-methoxybenzaldehyde as substrate, at pH 7.5 and 30°C
2
substrate hexanal, pH 6.0, 25°C
2.8
-
purified recombinant mutant S1M1
20.79
with 3-bromobenzaldehyde as substrate, at pH 7.5 and 30°C
21
with 4-bromobenzaldehyde as substrate, at pH 7.5 and 30°C
21.17
with 2-cyanobenzaldehyde as substrate, at pH 7.5 and 30°C
21.9
with hexanal as substrate, at pH 7.5 and 30°C
23.06
with 2-fluorobenzaldehyde as substrate, at pH 7.5 and 30°C
24.98
with propanal as substrate, at pH 7.5 and 30°C
26.46
with 4-methylbenzaldehyde as substrate, at pH 7.5 and 30°C
27.8
-
purified recombinant wild-type enzyme
3
substrate heptanal, pH 6.0, 25°C
3.2
substrate methyl glyoxal, pH 6.0, 25°C
3.3
substrate isovaleraldehyde, pH 6.0, 25°C
3.4
substrate methylglyoxal, pH 7.0, 25°C
3.5
purified recombinant enzyme, substrate 3,4-hexandione, pH 7.0, 30°C
3.8
with 2,4-dichlorobenzaldehyde as substrate, at pH 7.5 and 30°C
35
substrate methylglyoxal, pH 7.0, 25°C
4.14
with 3-hydroxybenzaldehyde and NADPH as substrates, at pH 7.0 and 30°C
4.2
substrate isovaleraldehyde, pH 6.0, 25°C
4.5
substrate p-anisaldehyde, pH 6.0, 25°C
4.65
-
after 9.7fold purification, at pH 7.0 and 25°C
4.7
substrate isoamyl pyruvate, pH 7.0, 25°C
42.7
with 1-butanal and NADH as substrates, at pH 7.0 and 30°C
5.3
substrate octanal, pH 6.0, 25°C
5.4
substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C
5.9
substrate valeraldehyde, pH 6.0, 25°C
50.1
substrate 2,3-hexanedione, pH 7.0, 25°C
6
substrate 2,3-butanedione, pH 7.0, 25°C
6.3
-
substrate 4-chlorobenzaldehyde, pH 6.5, temperature not specified in the publication
6.5
substrate 2,3-hexanedione, pH 7.0, 25°C
6.53
with 3-fluorobenzaldehyde as substrate, at pH 7.5 and 30°C
6.76
with 2-bromobenzaldehyde as substrate, at pH 7.5 and 30°C
6.9
substrate heptanal, pH 6.0, 25°C
60
substrate 2,3-butanedione, pH 7.0, 25°C
63.1
substrate isoamyl pyruvate, pH 7.0, 25°C
65
substrate 2,5-diketo-D-gluconate, pH 7.0, 25°C
69.6
substrate 2,3-pentanedione, pH 7.0, 25°C
7
-
substrate 4-fluorobenzaldehyde, pH 6.5, temperature not specified in the publication
74.7
substrate phenylglyoxal, pH 7.0, 25°C
75.4
substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C
8.33
with cinnamaldehyde and NADPH as substrates, at pH 7.0 and 30°C
80
purified recombinant enzyme, substrate 2,3-hexandione, pH 7.0, 30°C
89
purified recombinant enzyme, substrate 2,3-heptandione, pH 7.0, 30°C
9.9
-
purified recombinant mutant S1M4
92.8
substrate ethyl pyruvate, pH 7.0, 25°C
0.01
with acetophenone and NADH as substrates, at pH 7.0 and 30°C
0.01
with acetophenone and NADPH as substrates, at pH 7.0 and 30°C
0.01
with ethyl-4,4,4-trifloro-3-oxo-butanoate and NADPH as substrates, at pH 7.0 and 30°C
0.011
-
substrate 4-methylacetophenone, wild-type
0.011
-
substrate 4-tert-butylacetophenone, wild-type
0.02
with 2-oxo-2-phenylacetaldehyde and NADH as substrates, at pH 7.0 and 30°C
0.02
with ethyl-4,4,4-trifloro-3-oxo-butanoate and NADH as substrates, at pH 7.0 and 30°C
0.02
-
substrate 4-chloroacetophenone, wild-type
0.02
-
substrate 4-methoxyacetophenone, mutant Q245L
0.02
-
substrate 4-methoxyacetophenone, wild-type
0.02
-
substrate 4-methylacetophenone, mutant Q245L
0.025
-
substrate 4-fluoroacetophenone, mutant Q245P
0.025
-
substrate 4-methylacetophenone, mutant Q245H
0.58
with benzaldehyde and NADH as substrates, at pH 7.0 and 30°C
0.58
substrate benzaldehyde, pH 6.0, 25°C
1.2
substrate ethyl pyruvate, pH 7.0, 25°C
1.2
substrate furfural, pH 6.0, 25°C
2.1
substrate octanal, pH 6.0, 25°C
2.1
substrate valeraldehyde, pH 6.0, 25°C
33.96
with benzaldehyde and NADPH as substrates, at pH 7.0 and 30°C
33.96
with benzaldehyde as substrate, at pH 7.5 and 30°C
468
purified recombinant enzyme, substrate dihydroxyacetone, pH 7.0, 30°C
468
substrate dihydroxyacetone, 30°C, pH 7.0
7.9
pH 9.0, 55°C
7.9
purified recombinant His-tagged enzyme, pH 9.0, 70°C
additional information
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additional information
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additional information
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additional information
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additional information
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quantitative enzyme expression analysis in 59 samples of non-small-cell lung cancer, overview
additional information
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-
additional information
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enantiospecific assay
additional information
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enantiospecific assay
additional information
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plasma clearance of acetohexamide in male Wistar-Imamichi, showing very low enzyme amount, and Sprague-Dawley rats, overview
additional information
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-
additional information
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enantiospecific assay