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1.1.1.184: carbonyl reductase (NADPH)

This is an abbreviated version!
For detailed information about carbonyl reductase (NADPH), go to the full flat file.

Word Map on EC 1.1.1.184

Reaction

R-CHOH-R'
+
NADP+
=
R-CO-R'
+
NADPH
+
H+

Synonyms

(R) specific carbonyl reductase, (S)-specific carbonyl reductase, 15-hydroxyprostaglandin dehydrogenase [NADP+], 2,5-diketo-D-gluconic acid reductase, Adipocyte P27 protein, aldehyde reductase 1, aldehyde reductase I, aldo-keto reductase, ALR3, AP27, carbonyl reductase, carbonyl reductase (NADPH), carbonyl reductase 1, carbonyl reductase 3, carbonyl reductase S1, CBR, CBR 1, CBR 3, CBR1, CBR3, CHCR, CHCR1, CHCR2, CHCR3, CR, CR125, crc1, CSCR1, Gox0644, Gox1615, hCBR1, ketoreductase, KLCR1, KR, LCR, LOC415661, LOC610164, microsomal carbonyl reductase, More, NADP+-dependent ADH, NADPH-carbonyl reductase, NADPH-dependent carbonyl reductase, NADPH-dependent carbonyl reductase S1, NCCR, nonspecific NADPH-dependent carbonyl reductase, peroxisomal-type carbonyl reductase, PHCR, prostaglandin 9-ketoreductase, Prostaglandin-E2 9-reductase, PTCR, R-specific carbonyl reductase, reductase S1, reductase, carbonyl, RLCR, SCR, SCR9, SDR21C1, secondary-alcohol: NADP+-oxidoreductase, short-chain (S)-1-phenyl-1,2-ethanediol dehydrogenase, short-chain carbonyl reductase, sniffer, SRED, SSCR, SyS1, tetrameric carbonyl reductase, Tm1743, Tm_1743, xenobiotic carbonyl reductase, xenobiotic ketone reductase, YGL039w1, YGL039w2, YtbE, YueD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.184 carbonyl reductase (NADPH)

Specific Activity

Specific Activity on EC 1.1.1.184 - carbonyl reductase (NADPH)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.009
-
substrate 4-tert-butylacetophenone, mutant Q245L
0.011
0.013
-
substrate 4-bromoacetophenone, wild-type
0.014
-
substrate 4-fluoroacetophenone, wild-type
0.015 - 1.14
substrate specificity, overview
0.016
-
substrate 4-methoxyacetophenone, mutant Q245H
0.025
0.028
-
substrate acetophenone, wild-type
0.03
with cinnamaldehyde and NADH as substrates, at pH 7.0 and 30°C
0.032
-
substrate 4-tert-butylacetophenone, mutant Q245H
0.036
-
substrate 4-fluoroacetophenone, mutant Q245L
0.039
-
substrate acetophenone, mutant Q245P
0.045
-
substrate 4-methylacetophenone, mutant Q245P
0.047
-
substrate 4-bromoacetophenone, mutant Q245L
0.062
-
substrate 4-methoxyacetophenone, mutant Q245P
0.067
-
substrate 4-chloroacetophenone, mutant Q245L
0.072
-
substrate 4-fluoroacetophenone, mutant Q245H
0.073
-
substrate 1-phenylethanonee, pH 6.5, temperature not specified in the publication
0.074
-
substrate 1-(4-chlorophenyl)ethanone, pH 6.5, temperature not specified in the publication
0.084
-
substrate 4-tert-butylacetophenone, mutant Q245P
0.085
-
substrate acetophenone, mutant Q245H
0.086
-
substrate acetophenone, mutant Q245L
0.1
-
recombinant enzyme, substrate phenyl 3-oxobutyrate
0.12
-
recombinant enzyme, substrate trifluoromethyl 3-oxobutyrate
0.18
-
recombinant enzyme, substrate tert-butyl 3-oxobutyrate
0.2
-
recombinant enzyme, substrate ethyl 3-oxobutyrate
0.203
-
substrate 4-bromoacetophenone, mutant Q245H
0.238
-
substrate 4-chloroacetophenone, mutant Q245H
0.309
-
substrate 4-chloroacetophenone, mutant Q245P
0.403
-
substrate 4-bromoacetophenone, mutant Q245P
0.45
with 4-hydroxybenzaldehyde and NADPH as substrates, at pH 7.0 and 30°C
0.47
with 2-chlorobenzaldehyde and NADPH as substrates, at pH 7.0 and 30°C
0.48
-
crude extract, at pH 7.0 and 25°C
0.5
-
purified recombinant enzyme
0.522
-
substrate ethyl 3-oxopentanoate, pH 6.5, temperature not specified in the publication
0.6
substrate benzaldehyde, pH 6.0, 25°C
0.62
with 2-oxo-2-phenylacetic acid and NADPH as substrates, at pH 7.0 and 30°C
0.64
with ethyl-2-oxo-2-phenylacetate and NADH as substrates, at pH 7.0 and 30°C
0.65
substrate furfural, pH 6.0, 25°C
0.8
-
recombinant enzyme, substrate isopropyl 3-oxobutyrate
0.85
with ethyl-2-oxo-2-phenylacetate and NADPH as substrates, at pH 7.0 and 30°C
0.96
substrate methyl glyoxal, pH 6.0, 25°C
1.1
substrate p-anisaldehyde, pH 6.0, 25°C
1.4
-
recombinant enzyme, substrate chloromethyl 3-oxobutyrate
1.5
substrate hexanal, pH 6.0, 25°C
1.63
with 2-oxo-2-phenylacetaldehyde and NADPH as substrates, at pH 7.0 and 30°C
10.9
-
substrate 4-bromobenzaldehyde, pH 6.5, temperature not specified in the publication
12.6
-
pH 7.0, 30°C
125.3
Cylindrocarpon sclerotigenum
-
purified enzyme
13.35
with 4-nitrobenzaldehyde as substrate, at pH 7.5 and 30°C
1502
-
substrate ethyl 4-chloro-3-oxobutanoate, pH 6.5, temperature not specified in the publication
1533
substrate 2,3-butanedione, pH 7.4, 37°C
161.6
substrate daunorubicin, pH 7.4, 37°C
17
substrate 2,3-pentanedione, pH 7.0, 25°C
18.63
with 4-cyanobenzaldehyde as substrate, at pH 7.5 and 30°C
19.1
substrate phenylglyoxal, pH 7.0, 25°C
19.29
with pentanal as substrate, at pH 7.5 and 30°C
19.69
with 4-methoxybenzaldehyde as substrate, at pH 7.5 and 30°C
2
substrate hexanal, pH 6.0, 25°C
2.8
-
purified recombinant mutant S1M1
20.79
with 3-bromobenzaldehyde as substrate, at pH 7.5 and 30°C
21
with 4-bromobenzaldehyde as substrate, at pH 7.5 and 30°C
21.17
with 2-cyanobenzaldehyde as substrate, at pH 7.5 and 30°C
21.9
with hexanal as substrate, at pH 7.5 and 30°C
23.06
with 2-fluorobenzaldehyde as substrate, at pH 7.5 and 30°C
24.98
with propanal as substrate, at pH 7.5 and 30°C
26.46
with 4-methylbenzaldehyde as substrate, at pH 7.5 and 30°C
27.8
-
purified recombinant wild-type enzyme
3
substrate heptanal, pH 6.0, 25°C
3 - 8
-
purified enzyme
3.2
substrate methyl glyoxal, pH 6.0, 25°C
3.3
substrate isovaleraldehyde, pH 6.0, 25°C
3.4
substrate methylglyoxal, pH 7.0, 25°C
3.5
purified recombinant enzyme, substrate 3,4-hexandione, pH 7.0, 30°C
3.8
with 2,4-dichlorobenzaldehyde as substrate, at pH 7.5 and 30°C
33.96
35
substrate methylglyoxal, pH 7.0, 25°C
4.14
with 3-hydroxybenzaldehyde and NADPH as substrates, at pH 7.0 and 30°C
4.2
substrate isovaleraldehyde, pH 6.0, 25°C
4.5
substrate p-anisaldehyde, pH 6.0, 25°C
4.65
-
after 9.7fold purification, at pH 7.0 and 25°C
4.7
substrate isoamyl pyruvate, pH 7.0, 25°C
42.7
with 1-butanal and NADH as substrates, at pH 7.0 and 30°C
5.3
substrate octanal, pH 6.0, 25°C
5.4
substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C
5.9
substrate valeraldehyde, pH 6.0, 25°C
50.1
substrate 2,3-hexanedione, pH 7.0, 25°C
50.8
-
purified enzyme
6
substrate 2,3-butanedione, pH 7.0, 25°C
6.3
-
substrate 4-chlorobenzaldehyde, pH 6.5, temperature not specified in the publication
6.5
substrate 2,3-hexanedione, pH 7.0, 25°C
6.53
with 3-fluorobenzaldehyde as substrate, at pH 7.5 and 30°C
6.76
with 2-bromobenzaldehyde as substrate, at pH 7.5 and 30°C
6.9
substrate heptanal, pH 6.0, 25°C
60
substrate 2,3-butanedione, pH 7.0, 25°C
63.1
substrate isoamyl pyruvate, pH 7.0, 25°C
65
substrate 2,5-diketo-D-gluconate, pH 7.0, 25°C
69.6
substrate 2,3-pentanedione, pH 7.0, 25°C
7
-
substrate 4-fluorobenzaldehyde, pH 6.5, temperature not specified in the publication
74.7
substrate phenylglyoxal, pH 7.0, 25°C
75.4
substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C
75.9
-
pH 7.4, 30°C
8.33
with cinnamaldehyde and NADPH as substrates, at pH 7.0 and 30°C
80
purified recombinant enzyme, substrate 2,3-hexandione, pH 7.0, 30°C
89
purified recombinant enzyme, substrate 2,3-heptandione, pH 7.0, 30°C
9.9
-
purified recombinant mutant S1M4
92.8
substrate ethyl pyruvate, pH 7.0, 25°C
additional information