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1.1.1.184: carbonyl reductase (NADPH)

This is an abbreviated version!
For detailed information about carbonyl reductase (NADPH), go to the full flat file.

Word Map on EC 1.1.1.184

Reaction

R-CHOH-R'
+
NADP+
=
R-CO-R'
+
NADPH
+
H+

Synonyms

(R) specific carbonyl reductase, (S)-specific carbonyl reductase, 15-hydroxyprostaglandin dehydrogenase [NADP+], 2,5-diketo-D-gluconic acid reductase, Adipocyte P27 protein, aldehyde reductase 1, aldehyde reductase I, aldo-keto reductase, ALR3, AP27, carbonyl reductase, carbonyl reductase (NADPH), carbonyl reductase 1, carbonyl reductase 3, carbonyl reductase S1, CBR, CBR 1, CBR 3, CBR1, CBR3, CHCR, CHCR1, CHCR2, CHCR3, CR, CR125, crc1, CSCR1, Gox0644, Gox1615, hCBR1, ketoreductase, KLCR1, KR, LCR, LOC415661, LOC610164, microsomal carbonyl reductase, More, NADP+-dependent ADH, NADPH-carbonyl reductase, NADPH-dependent carbonyl reductase, NADPH-dependent carbonyl reductase S1, NCCR, nonspecific NADPH-dependent carbonyl reductase, peroxisomal-type carbonyl reductase, PHCR, prostaglandin 9-ketoreductase, Prostaglandin-E2 9-reductase, PTCR, R-specific carbonyl reductase, reductase S1, reductase, carbonyl, RLCR, SCR, SCR9, SDR21C1, secondary-alcohol: NADP+-oxidoreductase, short-chain (S)-1-phenyl-1,2-ethanediol dehydrogenase, short-chain carbonyl reductase, sniffer, SRED, SSCR, SyS1, tetrameric carbonyl reductase, Tm1743, Tm_1743, xenobiotic carbonyl reductase, xenobiotic ketone reductase, YGL039w1, YGL039w2, YtbE, YueD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.184 carbonyl reductase (NADPH)

Cloned

Cloned on EC 1.1.1.184 - carbonyl reductase (NADPH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 forms from rat testis
-
CBR1 coding regions excised from a pOTB7 recombinant plasmid and subcloned into pET28a vector. Constructs gave rise to a human CBR1 enzyme with a 6x-His tag separated by an 18-amino acid residue linker on the amino terminus. A factor Xa site inserted at the amino terminus between the linker and CBR1 gene. The pET28a constructs of the CBR1 wild-type and variant enzymes heat-shock transformed into Escherichia coli BL21 (DE3) pLysS-competent cells and expressed under the control of an IPTG-inducible T7 polymerase
CBR1 expressed as histidine-tagged protein
CBR3 expressed as histidine-tagged protein
co-expressed with glucose dehydrogenase from Exiguobacterium sibiricum in Escherichia coli BL21 (DE3) cells
-
co-expressed with glucose dehydrogenase from Thermus acidophilum in Escherichia coli BL21(DE3) cells
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
-
expressed from vector pET-22b in Escherichia coli BL21 (DE3)
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3)
expression in Sacchaormyces cerevisiae
expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3)
expression of isozymes CHCR1-3 as His-tagged proteins in Escherichia coli strain BL21(DE3)
-
expression of the heart enzyme in Escherichia coli BL21(DE3), assembly to tetrameric form
-
expression of wild-type and mutant enzymes in Escherichia coli strain HB101
-
expression of wild-type enzyme in Escherichia coli strain JM109, and of selenomethionine-CR in Escherichia coli strain B834
-
from a liver cDNA library, overexpression in Escherichia coli as His-tagged enzyme
-
from brain
-
functional overexpression as His-tagged protein in Escherichia coli BL21(DE3)
-
gene Gre2, high level expression in Escherichia coli strain BL21(DE3)
-
gene nccr, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
gene Tm1743, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
isozyme CBR3 V244M, genotyping and polymorphism distribution in 10 different ethnic groups, overview, expression of isozymes CBR V244 and CBR M244 in Escherichia coli strain BL21(DE3)
-
mutant enzyme V166A is co-expressed with alcohol dehydrogenase from Thermoanaerobacter ethanolicus in Rhodococcus opacus strain B-4
-
mutants overexpressed in Escherichia coli
mutation leads to change in coenzyme specificity
-
overexpression of SSCR in Escherichia coli strain BL21(DE3)
-
overview
pET28a-CHCR-transformed Escherichia coli BL21(DE3)pLys cells
quantitative enzyme expression analysis in 59 samples of non-small-cell lung cancer, overview
-
SCR overexpressed from pETSCR plasmid in Escherichia coli BL21 (DE3) as His6-tagged proteins
site directed mutagenesis
-
sniffer gene, expression as His-tagged enzyme from Escherichia coli strain M15
-
wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells
wild-type and mutant enzymes inserted into vector pQE30a and expressed in Escherichia coli BL21 (AITM) cells
wild-type and mutants expressed in Escherichia coli strain BL21(DE3) as His6-tagged proteins