1.1.1.179: D-xylose 1-dehydrogenase (NADP+, D-xylono-1,5-lactone-forming)

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For detailed information about D-xylose 1-dehydrogenase (NADP+, D-xylono-1,5-lactone-forming), go to the full flat file.

Reaction

Synonyms

D-xylose (nicotinamide adenine dinucleotide phosphate) dehydrogenase, D-xylose 1-dehydrogenase (NADP), D-xylose dehydrogenase, D-xylose-NADP dehydrogenase, D-xylose:NADP+ oxidoreductase, NADP+-dependent D-xylose dehydrogenase, NADP+:D-xylose dehydrogenase, XDH, xyd1

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.179 D-xylose 1-dehydrogenase (NADP+, D-xylono-1,5-lactone-forming)

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Results	in table
6	Activating Compound
213	AA Sequence
1	Application
1	CAS Registry Number
8	Cloned(Commentary)
25	Cofactor
1	Crystallization (Commentary)
6	Inhibitors
6	kcat/KM [mM/s]
51	KM Value [mM]
5	Metals/Ions
14	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
20	Organism
4	Pathway
1	PDB ID
12	pH Optimum
1	pH Range
20	Protein Variants
10	Purification (Commentary)
3	Reaction
4	Reaction Type
19	Reference
20	Source Tissue
11	Specific Activity [micromol/min/mg]
84	Substrates and Products (Substrate)
7	Subunits
10	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
23	Turnover Number [1/s]

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Cloned on EC 1.1.1.179 - D-xylose 1-dehydrogenase (NADP+, D-xylono-1,5-lactone-forming)

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cDNA subcloned in expression vector pRset, overexpression in Escherichia coli

Macaca fuscata

-

286127

cloned into pET19b, resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain

Haloferax volcanii

D4GP29, D4GP30

698940

expressed in Escherichia coli

Paenarthrobacter nicotinovorans

-

723749

expression in Escherichia coli

Haloferax volcanii

D4GP29, D4GP30

698940

expression in Kluyveromyces lactis

Trichoderma reesei

-

725867

gene xyd1, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in Saccharomyces cerevisiae

Trichoderma reesei

A8BT09

686797

overexpression in Escherichia coli

Haloarcula marismortui

Q5UY95

655884

wild-type protein and mutants are expressed in Escherichia coli BL21 (DE3)

Macaca fascicularis

Q9TQS6

684150