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1.1.1.169: 2-dehydropantoate 2-reductase

This is an abbreviated version!
For detailed information about 2-dehydropantoate 2-reductase, go to the full flat file.

Word Map on EC 1.1.1.169

Reaction

(R)-pantoate
+
NADP+
=
2-dehydropantoate
+
NADPH
+
H+

Synonyms

2-dehydropantoate 2-reductase, 2-ketopantoate reductase, 2-ketopantoic acid reductase, 2-oxopantoate reductase, conjugated polyketone reductase, CPR, ilvC, ketopantoate reductase, ketopantoic acid reductase, KPA reductase, KPR, More, PanE, panG, Tk-KPR, TK1968, TK1968 protein

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.169 2-dehydropantoate 2-reductase

Cloned

Cloned on EC 1.1.1.169 - 2-dehydropantoate 2-reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expressed with glucose dehydrogenase from Bacillus subtilis in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli BL21/lambdaDE3
-
expression in Escherichia coli K12
-
expression of wild-type and mutant enzymes
gene ilvC, complementation of an enzyme-deficient Escherichia coli mutant strain
-
gene panE, expression of His6-tagged wild-type and mutant enzymes
gene TK1968, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli
gene TK1968, recombinant coexpression of wild-type and mutant Tk-KPR enzyme dimers carrying a His6-tag and a Strep-tag on each monomer in Escherichia coli
gene TK1968, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta2 (DE3) pLysS
KPR expressed using a pRSETA vector in Escherichia coli strain BL21(DE3)C41
overexpression in strain BL21(DE3)
-
recombinant expression of C-terminally His-tagged wild-type and mutant enzymes