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1.1.1.159: 7alpha-hydroxysteroid dehydrogenase

This is an abbreviated version!
For detailed information about 7alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.159

Reaction

cholate
+
NAD+
=
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate
+
NADH
+
H+

Synonyms

7-alpha-HSDH, 7-HSDH, 7alpha-HSD, 7alpha-HSDH, 7alpha-hydroxy steroid dehydrogenase, 7alpha-hydroxysteroid dehydrogenase, Bile acid-inducible protein, CA 7alpha-HSDH, Ca7alpha-HSDH, dehydrogenase, 7alpha-hydroxy steroid, J-1-1, NADP(H)-dependent 7alpha-HSDH, NADP(H)-dependent 7alpha-hydroxysteroid dehydrogenase, NADP+-dependent 7alpha-HSDH, nicotinamide adenine dinucleotide phosphate-dependent 7alpha-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.159 7alpha-hydroxysteroid dehydrogenase

Reference

Reference on EC 1.1.1.159 - 7alpha-hydroxysteroid dehydrogenase

Please use the Reference Search for a specific query.
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Prabha, V.; Gupta, M.; Seiffge, D.; Gupta, K.G.
Purification of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli strain 080
Can. J. Microbiol.
36
131-135
1990
Escherichia coli
Manually annotated by BRENDA team
Prabha, V.; Gupta, M.; Gupta, K.G.
Kinetic properties of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli 080
Can. J. Microbiol.
35
1076-1080
1989
Escherichia coli
Manually annotated by BRENDA team
Ottolina, G.; Riva, S.; Carrea, G.; Danieli, B.; Buckmann, A.F.
Enzymatic synthesis of [4R-2H]NAD (P)H and [4S-2H]NAD(P)H and determination of the stereospecificity of 7alpha- and 12alpha hydroxysteroid dehydrogenase
Biochim. Biophys. Acta
998
173-178
1989
Escherichia coli
Manually annotated by BRENDA team
Kinoshita, S.; Kadota, K.; Inoue, T.; Sawada, H.; Taguchi, H.
Purification and properties of three kinds of alpha-hydroxysteroid dehydrogenase from Brevibacterium fuscum DC33
J. Ferment. Technol.
66
145-152
1988
Brevibacterium fuscum
-
Manually annotated by BRENDA team
MacDonald, I.A.; Roach, P.D.
Bile induction of 7alpha- and 7beta-hydroxysteroid dehydrogenases in Clostridium absonum
Biochim. Biophys. Acta
665
262-269
1981
Clostridium sardiniense
Manually annotated by BRENDA team
Haslewood, E.S.; Haslewood, G.A.D.
The specificity of a 7alpha-hydroxy steroid dehydrogenase from Escherichia coli
Biochem. J.
157
207-210
1976
Escherichia coli
Manually annotated by BRENDA team
Hylemon, P.B.; Sherrod, J.A.
Multiple forms of 7-alpha-hydroxysteroid dehydrogenase in selected strains of Bacteroides fragilis
J. Bacteriol.
122
418-424
1975
Bacteroides fragilis
Manually annotated by BRENDA team
MacDonald, I.A.; Williams, C.N.; Mahony, D.E.; Christie, W.M.
NAD- and NADP-dependent 7alpha-hydroxysteroid dehydrogenases from bacteroides fragilis
Biochim. Biophys. Acta
384
12-24
1975
Bacteroides fragilis
Manually annotated by BRENDA team
MacDonald, I.A.; Williams, C.N.; Mahony, D.E.
7alpha-hydroxysteroid dehydrogenase from Escherichia coli B: preliminary studies
Biochim. Biophys. Acta
309
243-253
1973
Escherichia coli
Manually annotated by BRENDA team
Tanaka, N.; Nonaka, T.; Tanabe, T.; Yoshimoto, T.; Tsuru, D.; Mitsui, Y.
Crystal structures of the binary and ternary complexes of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli
Biochemistry
35
7715-7730
1996
Escherichia coli (P0AET8), Escherichia coli
Manually annotated by BRENDA team
Baron, S.F.; Franklund, C.V.; Hylemon, P.B.
Cloning, sequencing, and expression of the gene coding for bile acid 7alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708
J. Bacteriol.
173
4558-4569
1991
Eubacterium sp., Eubacterium sp. VPI 12708
Manually annotated by BRENDA team
Tanabe, T.; Tanaka, N.; Uchikawa, K.; Kabashima, T.; Ito, K.; Nonaka, T.; Mitsui, Y.; Tsuru, M.; Yoshimoto, T.
Roles of the Ser146, Tyr159, and Lys163 residues in the catalytic action of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli
J. Biochem.
124
634-641
1998
Escherichia coli
Manually annotated by BRENDA team
Coleman, J.P.; Hudson, L.L.; Adams, M.J.
Characterization and regulation of the NADP-linked 7alpha-hydroxysteroid dehydrogenase gene from Clostridium sordellii
J. Bacteriol.
176
4865-4874
1994
Paeniclostridium sordellii
Manually annotated by BRENDA team
Yoshimoto, T.; Higashi, H.; Kanatani, A.; Lin, X.S.; Nagai, H.; Oyama, H.; Kurazono, K.; Tsuru, D.
Cloning and sequencing of the 7alpha-hydroxysteroid dehydrogenase gene from Escherichia coli HB101 and characterization of the expressed enzyme
J. Bacteriol.
173
2173-2179
1991
Escherichia coli
Manually annotated by BRENDA team
Bennett, M.J.; McKnight, S.L.; Coleman, J.P.
Cloning and characterization of the NAD-dependent 7alpha-Hydroxysteroid dehydrogenase from Bacteroides fragilis
Curr. Microbiol.
47
475-484
2003
[Clostridium] scindens, Paeniclostridium sordellii, Escherichia coli, Bacteroides fragilis (Q9S3U5), Bacteroides fragilis
Manually annotated by BRENDA team
Ueda, S.; Oda, M.; Imamura, S.; Ohnishi, M.
Molecular and enzymatic properties of 7alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831
J. Biol. Macromol.
4
33-38
2004
Pseudomonas sp.
-
Manually annotated by BRENDA team
Pedrini, P.; Andreotti, E.; Guerrini, A.; Dean, M.; Fantin, G.; Giovannini, P.P.
Xanthomonas maltophilia CBS 897.97 as a source of new 7beta- and 7alpha-hydroxysteroid dehydrogenases and cholylglycine hydrolase: improved biotransformations of bile acids
Steroids
71
189-198
2006
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia CBS 897.97
Manually annotated by BRENDA team
Zhu, D.; Stearns, J.E.; Ramirez, M.; Hua, L.
Enzymatic enantioselective reduction of alpha-ketoesters by a thermostable 7alpha-hydroxysteroid dehydrogenase from Bacteroides fragilis
Tetrahedron
62
4535-4539
2006
Bacteroides fragilis
-
Manually annotated by BRENDA team
Fukiya, S.; Arata, M.; Kawashima, H.; Yoshida, D.; Kaneko, M.; Minamida, K.; Watanabe, J.; Ogura, Y.; Uchida, K.; Itoh, K.; Wada, M.; Ito, S.; Yokota, A.
Conversion of cholic acid and chenodeoxycholic acid into their 7-oxo derivatives by Bacteroides intestinalis AM-1 isolated from human feces
FEMS Microbiol. Lett.
293
263-270
2009
Bacteroides fragilis, Bacteroides intestinalis, Escherichia coli HB101, Bacteroides fragilis NCTC 9343, Bacteroides intestinalis am-1
Manually annotated by BRENDA team
Giovannini, P.P.; Grandini, A.; Perrone, D.; Pedrini, P.; Fantin, G.; Fogagnolo, M.
7alpha- and 12alpha-Hydroxysteroid dehydrogenases from Acinetobacter calcoaceticus lwoffii: a new integrated chemo-enzymatic route to ursodeoxycholic acid
Steroids
73
1385-1390
2008
Acinetobacter lwoffii
Manually annotated by BRENDA team
Eggert, T.; Bakonyi, D.; Hummel, W.
Enzymatic routes for the synthesis of ursodeoxycholic acid
J. Biotechnol.
191
11-21
2014
Acinetobacter calcoaceticus, Bacteroides fragilis, Escherichia coli, Pseudomonas sp., Stenotrophomonas maltophilia, Clostridium sardiniense (G9FRD7)
Manually annotated by BRENDA team
Ji, W.; Chen, Y.; Zhang, H.; Zhang, X.; Li, Z.; Yu, Y.
Cloning, expression and characterization of a putative 7alpha-hydroxysteroid dehydrogenase in Comamonas testosteroni
Microbiol. Res.
169
148-154
2014
Comamonas testosteroni (S5U393), Comamonas testosteroni, Comamonas testosteroni ATCC 11996 (S5U393)
Manually annotated by BRENDA team
Lou, D.; Wang, B.; Tan, J.; Zhu, L.
Carboxyl-terminal and Arg38 are essential for activity of the 7alpha-hydroxysteroid dehydrogenase from Clostridium absonum
Protein Pept. Lett.
21
894-900
2014
Clostridium sardiniense (G9FRD7), Clostridium sardiniense
Manually annotated by BRENDA team
Yang, Q.; Li, L.; Wang, B.; Zhu, L.; Tan, J.
Modifying the microenvironment of epoxy resin to improve the activity of immobilized 7alpha-hydroxysteroid dehydrogenases
Appl. Biochem. Biotechnol.
193
925-939
2020
Clostridium sardiniense (G9FRD7)
Manually annotated by BRENDA team
Huang, B.; Zhao, Q.; Zhou, J.H.; Xu, G.
Enhanced activity and substrate tolerance of 7alpha-hydroxysteroid dehydrogenase by directed evolution for 7-ketolithocholic acid production
Appl. Microbiol. Biotechnol.
103
2665-2674
2019
Clostridium sardiniense (G9FRD7), Clostridium sardiniense DSM 1599 (G9FRD7)
Manually annotated by BRENDA team
Zhang, X.; Fan, D.; Hua, X.; Zhang, T.
Large-scale production of ursodeoxycholic acid from chenodeoxycholic acid by engineering 7alpha- and 7beta-hydroxysteroid dehydrogenase
Bioprocess Biosyst. Eng.
42
1537-1545
2019
Clostridium sardiniense (G9FRD7), Clostridium sardiniense DSM 599 (G9FRD7)
Manually annotated by BRENDA team
Lou, D.; Wang, Y.; Tan, J.; Zhu, L.; Ji, S.; Wang, B.
Functional contribution of coenzyme specificity-determining sites of 7alpha-hydroxysteroid dehydrogenase from Clostridium absonum
Comput. Biol. Chem.
70
89-95
2017
Clostridium sardiniense (G9FRD7)
Manually annotated by BRENDA team
Ji, S.; Pan, Y.; Zhu, L.; Tan, J.; Tang, S.; Yang, Q.; Zhang, Z.; Lou, D.; Wang, B.
A novel 7alpha-hydroxysteroid dehydrogenase Magnesium ion significantly enhances its activity and thermostability
Int. J. Biol. Macromol.
177
111-118
2021
unidentified microorganism
Manually annotated by BRENDA team