1.1.1.141: 15-hydroxyprostaglandin dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about 15-hydroxyprostaglandin dehydrogenase (NAD+), go to the full flat file.

Reaction

Synonyms

11alpha,15-dihydroxy-9-oxoprost-13-enoate:NAD+ 15-oxidoreductase, 15-hydroxy prostaglandin dehydrogenase, 15-hydroxy-prostaglandin-dehydrogenase, 15-hydroxyprostaglandin dehydrogenase, 15-hydroxyprostaglandin-dehydrogenase, 15-hydroxyprostanoic dehydrogenase, 15-OH-PGDH, 15-PGDH, 15-prostaglandin dehydrogenase, dehydrogenase, 15-hydroxyprostaglandin, HPGD, NAD+ dependent 15-hydroxyprostaglandin dehydrogenase, NAD+ dependent PGDH, NAD+-15-hydroxy prostanoate oxidoreductase, NAD+-dependent 15-hydroxyprostaglandin dehydrogenase, NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (type I), NAD+-dependent 15-PGDH, NAD+-linked 15-hydroxyprostaglandin dehydrogenase, NAD-dependent 15-hydroxyprostaglandin dehydrogenase, NAD-specific 15-hydroxyprostaglandin dehydrogenase, nicotinamide adenine dinucleotide-dependent 15-hydroxyprostaglandin dehydrogenase, PGDH, prostaglandin dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.141 15-hydroxyprostaglandin dehydrogenase (NAD+)

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Results	in table
30	Activating Compound
43	AA Sequence
9	Application
1	CAS Registry Number
23	Cloned(Commentary)
62	Cofactor
1	Crystallization (Commentary)
1263	Disease/ Diagnostics
11	Expression
9	General Information
7	General Stability
108	IC50 Value
268	Inhibitors
7	kcat/KM [mM/s]
38	Ki Value [mM]
100	KM Value [mM]
8	Localization
19	Molecular Weight [Da]
51	Natural Substrates/ Products (Substrates)
164	Organism
6	Pathway
1	PDB ID
19	pH Optimum
2	pH Range
70	Protein Variants
34	Purification (Commentary)
9	Reaction
3	Reaction Type
101	Reference
226	Source Tissue
24	Specific Activity [micromol/min/mg]
8	Storage Stability
175	Substrates and Products (Substrate)
14	Subunits
121	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
4	Temperature Stability [°C]
25	Turnover Number [1/s]

KM Value

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KM Value on EC 1.1.1.141 - 15-hydroxyprostaglandin dehydrogenase (NAD+)

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0012 - 0.026

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

16 entries

0.00765 - 0.012

(5Z,8E,10E,12S)-12-hydroxy-5,8,10-heptadecatrienoic acid

2 entries

0.016

15-hydroxyeicosatetraenoic acid

Oryctolagus cuniculus

-

-

285938

0.0521

15-ketoprostaglandin E1

Homo sapiens

-

at pH 7.0

285916

0.0266 - 0.0528

15-ketoprostaglandin E2

2 entries

0.077

19-hydroxyprostaglandin E1

Oryctolagus cuniculus

-

-

285938

0.283

20-hydroxyprostaglandin E1

Oryctolagus cuniculus

-

-

285938

0.066

6-ketoprostaglandin E1

Oryctolagus cuniculus

-

-

285921

0.111

6-ketoprostaglandin F1alpha

Oryctolagus cuniculus

-

-

285921

0.063

6-oxo-prostaglandin F1alpha

Homo sapiens

-

recombinant enzyme expressed in E. coli

285937

0.022 - 0.72

NAD+

24 entries

0.0156 - 0.0594

NADH

3 entries

0.0045 - 0.038

prostaglandin A1

3 entries

0.003 - 0.022

Prostaglandin A2

2 entries

0.741

Prostaglandin D2

Oryctolagus cuniculus

-

-

285921, 285938

0.0013 - 0.033

prostaglandin E1

10 entries

0.00158 - 0.059

prostaglandin E2

17 entries

0.077

prostaglandin E3

Oryctolagus cuniculus

-

-

285938

0.0213 - 0.133

prostaglandin F2alpha

5 entries

additional information

additional information

Homo sapiens

-

Michaelis-Menten kinetics, overview

285916

-

0.0012

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, I17L mutant

654374

0.0026

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, N91D mutant

654374

0.0034

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5

654374

0.0035

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, V186K mutant

654374

0.0039

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

-

654158

0.0045

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, V186A mutant

654374

0.006

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, N91A mutant

654374

0.0063

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5

654361

0.0071

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5

657209

0.0086

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, T11S mutant

657209

0.01

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Cavia porcellus

-

pH 7.4, 37°C

285918

0.011

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, I17A mutant

654374

0.011

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, I17V mutant

654374

0.013

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, Q15K/W37K double mutant

654361

0.022

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, V186I mutant

654374

0.026

(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate

Homo sapiens

-

37°C, pH 7.5, Q15K mutant

654361

0.00765

(5Z,8E,10E,12S)-12-hydroxy-5,8,10-heptadecatrienoic acid

Homo sapiens

-

-

285935

0.012

(5Z,8E,10E,12S)-12-hydroxy-5,8,10-heptadecatrienoic acid

Oryctolagus cuniculus

-

-

285938

0.0266

15-ketoprostaglandin E2

Homo sapiens

-

at pH 9.0

285916

0.0528

15-ketoprostaglandin E2

Homo sapiens

-

at pH 7.0

285916

0.022

NAD+

Homo sapiens

-

recombinant enzyme expressed in E. coli

285937

0.022

NAD+

Homo sapiens

-

37°C, pH 7.5, V186K mutant

654374

0.0227

NAD+

Homo sapiens

-

at pH 9.0, + prostaglandin E2

285916

0.024

NAD+

Homo sapiens

-

37°C, pH 7.5, V186A mutant

654374

0.0284

NAD+

Homo sapiens

-

at pH 7.0, + prostaglandin E2

285916

0.029

NAD+

Homo sapiens

-

at pH 9.0, + prostaglandin E1

285916

0.03

NAD+

Homo sapiens

-

37°C, pH 7.5

657209

0.0308

NAD+

Homo sapiens

-

at pH 7.0, + prostaglandin E1

285916

0.032

NAD+

Homo sapiens

-

37°C, pH 7.5

654361

0.032

NAD+

Homo sapiens

-

37°C, pH 7.5, N91D mutant

654374

0.038

NAD+

Homo sapiens

-

37°C, pH 7.5

654374

0.0384

NAD+

Sus scrofa

-

-

285924

0.0399

NAD+

Homo sapiens

-

+ prostaglandin F2alpha

285920

0.0405

NAD+

Homo sapiens

-

+ prostaglandin E2

285920

0.0451

NAD+

Homo sapiens

-

+ prostaglandin E1

285920

0.046

NAD+

Homo sapiens

-

-

654158

0.059

NAD+

Homo sapiens

-

37°C, pH 7.5, I17L mutant

654374

0.098

NAD+

Homo sapiens

-

37°C, pH 7.5, I17V mutant

654374

0.11

NAD+

Bos taurus

-

-

285926

0.117

NAD+

Homo sapiens

-

-

285935

0.27

NAD+

Homo sapiens

-

37°C, pH 7.5, V186I mutant

654374

0.55

NAD+

Homo sapiens

-

37°C, pH 7.5, I17A mutant

654374

0.7

NAD+

Homo sapiens

-

37°C, pH 7.5, T11S mutant

657209

0.72

NAD+

Homo sapiens

-

37°C, pH 7.5, N91A mutant

654374

0.0156

NADH

Homo sapiens

-

at pH 7.0, + 15-keto-PGE1

285916

0.0156

NADH

Homo sapiens

-

at pH 7.0, + 15-ketoprostaglandin E2

285916

0.0594

NADH

Homo sapiens

-

at pH 9.0, + 15-ketoprostaglandin E2

285916

0.0045

prostaglandin A1

Homo sapiens

-

recombinant enzyme expressed in E. coli

285937

0.033

prostaglandin A1

Homo sapiens

-

-

285927

0.038

prostaglandin A1

Oryctolagus cuniculus

-

-

285921, 285938

0.003

Prostaglandin A2

Homo sapiens

-

-

285927

0.022

Prostaglandin A2

Homo sapiens

-

recombinant enzyme expressed in E. coli

285937

0.0013

prostaglandin E1

Homo sapiens

-

-

285923

0.0013

prostaglandin E1

Homo sapiens

-

at pH 7.0

285916

0.0034

prostaglandin E1

Bos taurus

-

-

285926

0.0048

prostaglandin E1

Homo sapiens

-

-

285923

0.0048

prostaglandin E1

Homo sapiens

-

at pH 9.0

285916

0.005

prostaglandin E1

Homo sapiens

-

recombinant enzyme expressed in E. coli

285937

0.0054

prostaglandin E1

Homo sapiens

-

-

285920

0.027

prostaglandin E1

Homo sapiens

-

-

285927

0.033

prostaglandin E1

Oryctolagus cuniculus

-

-

285921, 285938

0.033

prostaglandin E1

Oryctolagus cuniculus

-

pH 10.2

285921

0.00158

prostaglandin E2

Sus scrofa

-

-

285924

0.0025

prostaglandin E2

Homo sapiens

-

at pH 7.0

285916

0.0026

prostaglandin E2

Homo sapiens

-

-

285920

0.0028

prostaglandin E2

Homo sapiens

-

at pH 9.0

285916

0.0028

prostaglandin E2

Homo sapiens

-

pH 8.0, 25°C, 20 nM [1-(3-methylphenyl)-1H-benzimidazol-5-yl](piperidin-1-yl)methanone

726267

0.0037

prostaglandin E2

Homo sapiens

-

pH 8.0, 25°C, 10 nM [1-(3-methylphenyl)-1H-benzimidazol-5-yl](piperidin-1-yl)methanone

726267

0.0039

prostaglandin E2

Homo sapiens

-

recombinant enzyme expressed in E. coli

285937

0.0055

prostaglandin E2

Homo sapiens

-

pH 8.0, 25°C

726267

0.0056

prostaglandin E2

Homo sapiens

-

pH 8.0, 25°C, 4 nM 3-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepine

726267

0.0064

prostaglandin E2

Homo sapiens

-

pH 8.0, 25°C, 10 nM 3-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepine

726267

0.0075

prostaglandin E2

Homo sapiens

-

pH 8.0, 25°C, 10 nM 2-([(6-bromo-4H-imidazo[4,5-b]pyridin-2-yl)sulfanyl]methyl)benzonitrile

726267

0.01

prostaglandin E2

Homo sapiens

-

-

285927, 285935

0.0117

prostaglandin E2

Homo sapiens

-

-

285933

0.0135

prostaglandin E2

Homo sapiens

-

pH 8.0, 25°C, 10 nM 2-([(6-bromo-4H-imidazo[4,5-b]pyridin-2-yl)sulfanyl]methyl)benzonitrile

726267

0.014

prostaglandin E2

Gallus gallus

-

-

285912

0.059

prostaglandin E2

Oryctolagus cuniculus

-

-

285921, 285938

0.059

prostaglandin E2

Oryctolagus cuniculus

-

pH 10.2

285921

0.0213

prostaglandin F2alpha

Homo sapiens

-

-

285920

0.025

prostaglandin F2alpha

Gallus gallus

-

-

285912

0.04

prostaglandin F2alpha

Homo sapiens

-

recombinant enzyme expressed in E. coli

285937

0.059

prostaglandin F2alpha

Homo sapiens

-

-

285927

0.133

prostaglandin F2alpha

Oryctolagus cuniculus

-

-

285921, 285938