1.1.1.138: mannitol 2-dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about mannitol 2-dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

Cla h 8, Cla h 8 allergen, D-mannitol 2-dehydrogenase, D-mannitol dehydrogenase, D-mannitol dehydrogenase, NADP-dependent, Gox1432, LRMDH, LXR1, MAD1p, mannitol 2-dehydrogenase, Mannitol 2-dehydrogenase [NADP+], mannitol dehydrogenase, mannitol dehydrogenase (NADP+), mannitol dehydrogenase (nicotinamide dinucleotide phosphate), mannitol dehydrogenase 1, MDH, More, MtDH, NADP+-dependent mannitol dehydrogenase, NADP-dependent mannitol dehydrogenase, NADP-dependent MtDH, NADP-mannitol dehydrogenase, PIG8p, PSLDR, TbMDH

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.138 mannitol 2-dehydrogenase (NADP+)

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Results	in table
5	Activating Compound
26	AA Sequence
6	Application
1	CAS Registry Number
11	Cloned(Commentary)
59	Cofactor
6	Crystallization (Commentary)
2	Expression
3	General Information
49	Inhibitors
3	kcat/KM [mM/s]
6	Ki Value [mM]
69	KM Value [mM]
1	Localization
12	Metals/Ions
31	Molecular Weight [Da]
47	Natural Substrates/ Products (Substrates)
56	Organism
2	Pathway
28	PDB ID
25	pH Optimum
5	pH Range
3	pH Stability
4	pI Value
4	Protein Variants
16	Purification (Commentary)
3	Reaction
3	Reaction Type
48	Reference
38	Source Tissue
22	Specific Activity [micromol/min/mg]
3	Storage Stability
104	Substrates and Products (Substrate)
21	Subunits
43	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]

Cloned

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Cloned on EC 1.1.1.138 - mannitol 2-dehydrogenase (NADP+)

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Agaricus bisporus

-

285871

DNA and amino acid sequence determination and analysis, the enzyme shows a carbon responsive transcriptional pattern and is up-regulated when mycelia are transferred to a culture medium containing D-mannitol or D-fructose, quantitative expression and phylogenetic analysis

Tuber borchii

Q1ACW3

686917

expressed in E. coli

Cladosporium herbarum

P0C0Y5

669463

expressed in Escherichia coli

Alternaria alternata

P0C0Y4

668299

expressed in Escherichia coli DH5-alpha cells

Gluconobacter oxydans

-

739923

expression in Escherichia coli

Agaricus bisporus

-

285870

expression in Pichia pastoris, gene cloning in Escherichia coli

Agaricus bisporus

-

285854

expression of mutant enzymes in Escherichia coli strain JM109

Pseudomonas fluorescens

-

686764

gene MAD1, expression analysis in fungal tissue, recombinant expression in yeast

Uromyces viciae-fabae

-

670585

gene mdh, DNA and amino acid sequence determination and compiational amnipulation, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Limosilactobacillus reuteri

-

667280

gene MtDH, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Cladosporium herbarum

P0C0Y5

669463