1.1.1.121: aldose 1-dehydrogenase (NAD+)
This is an abbreviated version!
For detailed information about aldose 1-dehydrogenase (NAD+), go to the full flat file.
Word Map on EC 1.1.1.121
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1.1.1.121
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electrode
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biosensors
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gluconobacter
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osmium
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ferrocene
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cathode
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nadp+
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acidophilum
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pqq-dependent
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thermophilus
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archaeon
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voltage
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mass-producible
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oxydans
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thermoplasma
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d-fucose
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thermoacidophilic
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electrochemical
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anode
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biofuel
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printed
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xylose
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benchmark
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graphite
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laccase
- 1.1.1.121
-
electrode
-
biosensors
- gluconobacter
-
osmium
- ferrocene
-
cathode
- nadp+
- acidophilum
-
pqq-dependent
- thermophilus
- archaeon
-
voltage
-
mass-producible
- oxydans
-
thermoplasma
- d-fucose
-
thermoacidophilic
-
electrochemical
-
anode
-
biofuel
-
printed
- xylose
-
benchmark
-
graphite
- laccase
Reaction
Synonyms
aldohexose dehydrogenase, aldose dehydrogenase, aldose-1-dehydrogenase, AldT, D-aldohexose dehydrogenase, dehydrogenase, D-aldohexose, More, Ta0754, TAD, TTHA0369
ECTree
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Temperature Stability
Temperature Stability on EC 1.1.1.121 - aldose 1-dehydrogenase (NAD+)
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55
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half-life: 40 s in sodium phosphate buffer, pH 7.0, with D-fucose, D-galactose, D-glucose or D-mannose as substrate
85
90
15 min, wild-type enzyme loses about 10% of its activity, mutant enzyme F141A loses about 25% of activity, mutant enzyme F249A loses about 25% of activity, mutant enzyme D242N completely loses activity
95
15 min, wild-type enzyme loses about 40% of its activity, mutant enzyme F141A loses about 50% of activity, mutant enzyme F249A loses about 70% of activity, mutant enzyme D242N completely loses activity
15 min, wild-type enzyme is stable, mutant enzyme F141A loses about 5% of activity, mutant enzyme F249A loses about 10% of activity, mutant enzyme D242N loses 60% of activity
85
approximately 40% of the activity is inactivated by the incubation of the enzyme solution (0.3 mg/ml in 10 mM potassium phosphate, pH 7.0) for 15 min at 95°C