1.1.1.105: all-trans-retinol dehydrogenase (NAD+)

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For detailed information about all-trans-retinol dehydrogenase (NAD+), go to the full flat file.

Reaction

Synonyms

ADH, alcohol dehydrogenase, all-trans retinol dehydrogenase, all-trans-retinol dehydrogenase, dehydrogenase, retinol, DHRS9, epidermal retinol dehydrogenase 2, LOC100185899, MDR, microsomal retinol dehydrogenase, mRDH1, NAD+-dependent retinoid-active short-chain dehydrogenase/reductase, P32, PDHC, photoreceptor retinol dehydrogenase isoform C, RDH-E2, RDH-like SDR, Rdh1, RDH10, RDH10a, RDH10b, RDH10c, RDH12, RDH16, RDH2, RDH7, RDH8, RDHE2, RDHE2S, RDHL, retinal reductase, retinene reductase, retinoid dehydrogenase/reductase, retinol dehydrogenase (vitamin A1), retinol dehydrogenase 10, retinol dehydrogenase 12, retinol dehydrogenase 2, retinol dehydrogenase 4, retinol dehydrogenase epidermal 2, retinol dehydrogenase epidermal 2-similar, RL-HSD, RoDH-4, RoDH-like 3alpha-hydroxysteroid dehydrogenase, RoDH4, SDR16C5, SDR16C6, XP_2120364

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.105 all-trans-retinol dehydrogenase (NAD+)

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Results	in table
2	Activating Compound
166	AA Sequence
2	Application
1	CAS Registry Number
24	Cloned(Commentary)
58	Cofactor
1	Crystallization (Commentary)
509	Disease/ Diagnostics
4	Expression
29	General Information
3	General Stability
17	Inhibitors
1	kcat/KM [mM/s]
7	Ki Value [mM]
31	KM Value [mM]
23	Localization
2	Metals/Ions
8	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
53	Organism
3	Pathway
4	PDB ID
5	pH Optimum
1	pH Range
2	Posttranslational Modification
24	Protein Variants
7	Purification (Commentary)
1	Reaction
3	Reaction Type
43	Reference
62	Source Tissue
5	Specific Activity [micromol/min/mg]
1	Storage Stability
101	Substrates and Products (Substrate)
7	Subunits
85	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.105 - all-trans-retinol dehydrogenase (NAD+)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

immobilized metal ion affinity chromatography

Kangiella koreensis

C7R702

737923

Ni2+-NTA resin column chromatography

Homo sapiens

Q8N3Y7

691750

preparation of RoDH-4 contains a minor contaminating protein band. Therefore, the exact concentration of RoDH-4 is determined based on the calibration curve constructed from several concentrations of BSA loaded on to the same SDS/PAGE gel

Homo sapiens

O75452

667537

RDH1 activity during purification is maintained only with phosphotidylcholine

Mus musculus

-

721724

recombinant enzyme

Homo sapiens

Q8N3Y7

691750

Sephacryl S300 gel filtration

Drosophila melanogaster

Q7KNR7

740085

solubilized enzyme is extremely unstable and loses almost 80% of the initial activity even at partially purified stage, purification uses resistance of enzyme to proteolysis at 4°C in the presence of 1% Triton

Homo sapiens

-

654713