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1.1.1.103: L-threonine 3-dehydrogenase

This is an abbreviated version!
For detailed information about L-threonine 3-dehydrogenase, go to the full flat file.

Reaction

L-threonine
+
NAD+
=
L-2-amino-3-oxobutanoate
+
NADH
+
H+

Synonyms

CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.103 L-threonine 3-dehydrogenase

Cloned

Cloned on EC 1.1.1.103 - L-threonine 3-dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned as a 3562-base pair EcoRI fragment
-
DNA and amino acid seuence determination and analysis, sequence comparisons, phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strains JM109 and BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3)
gene tdh, functional expression in Escherichia coli
-
into pET-30a digested with KpnI–EcoRI to construct pET-ste11 and expressed in Escherichia coli BL21
-
ligated into the pTZ57R/T cloning vector. The recombinant pTZ-tdh plasmid digested with NcoI and EcoRI and the TDH gene ligated with pET-8c, giving the recombinant pET-tdh plasmid, used to transform Escherichia coli DH5alpha competent cells. Recombinant and purified pET-tdh plasmid expressed in Escherichia coli BL21 (DE3)
orf PH0655, amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain TOP10
-
orf PH0655, expression in Escherichia coli
-
orf PH0655, gene TDH, overexpression in Escherichia coli strain BL21(DE3)
-
orf PH0655, NA and amino acid sequence determination, analysis, and comparison, expression of selenomethionine-labeled wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
orf PH0655, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
overexpression in Escherichia coli
-
plasmid pTZ-tdh transformed in Escherichia coli DH5alpha competent cells. NcoI-EcoRI restriction fragment inserted into the pET-8c expression vector at the corresponding sites. The resulting plasmid pET-tdh expressed in Escherichia coli strain BL21(DE3)CodonPlus-RIL
-
recombinant pET-tdh plasmid expressed in Escherichia coli BL21 (DE3)